短雄花序板栗芽变的AFLP分析  

AFLP Analysis on the Short Catkins Mutation of Chestnut

作　　者：徐月[1] 曹庆芹[2] 冯永庆[1] 杨凯[2] 秦岭[1] 廖康[3] 

机构地区：[1]北京农学院植物科学技术系,北京102206 [2]北京农学院生物技术系,北京102206 [3]新疆农业大学园艺学院,新疆乌鲁木齐830052

出　　处：《园艺学报》2006年第6期1321-1324,共4页Acta Horticulturae Sinica

基　　金：北京市自然科学基金资助项目(6052004);北京市属市管高校人才强教计划资助项目

摘　　要：利用AFLP分子标记技术对板栗短雄花序芽变及其母株进行了初步研究,72种引物组合共产生5129条清晰谱带,多态性位点53个,多态性2.05%,相似性系数0.9897,差异片段主要集中于300～900bp之间。研究表明短雄花序芽变和对照个体之间变异与遗传物质的改变相关,其中一些可能与雄花序变短的基因有关。AFLP was used to compare the short catkins sport and its mother plant in chestnut （ Castanea mollissima Bl. ）. A total of 72 selective primer pairs amplified 5 129 clear bands, of those 53 were polymorphic loci. The polymorphic rate and Nei＇s association coefficient between them were 2. 05% and 0. 9897 respectively. The molecular weight range of the polymorphic fragments was mainly 300 -900 bp. The results indicated that there were some molecular-level differentiation between chestnut and its short catkins sport. Some of the specific segments had been considered as markers of short catkins in chestnut.

关 键 词：板栗 芽变 短雄花序 AFLP 

分 类 号：S664.2[农业科学—果树学]