嗜水气单胞菌感染后牛蛙血清中抗菌物质的初步研究  

Studies on antibacterial protein of bullfrog serum infected by Aeromonas hydrophila

作　　者：邹文政[1] 张俊杰[1] 鄢庆枇[1] 纪荣兴[1] 

机构地区：[1]集美大学水产学院,福建厦门361021

出　　处：《水产学报》2007年第1期62-67,共6页Journal of Fisheries of China

基　　金：福建省自然科学基金(B0410022)

摘　　要：用1×107mL-1的嗜水气单胞菌腹腔注射感染牛蛙,注射后7d内试验组牛蛙血清抗菌活性都高于对照组,其中在3d、7d时存在显著性差异,峰值出现在感染后第3天。收集第2批人工感染后3d时牛蛙的血清。该血清经Sephadex G-25凝胶柱分离后,并在波长280nm处测定紫外吸收值,得到两个吸收峰。以嗜水气单胞菌检测分离物的抗菌活性,结果表明经诱导的血清抗菌物质主要集中在第1个吸收峰。通过对金黄色葡萄球菌等10株指示菌抗菌谱的测定,其对大肠杆菌、枯草芽孢杆菌、铜绿假单胞菌等具有明显的抗菌作用;SDS-PAGE分析显示抗菌蛋白分子量较大。结果说明:牛蛙被嗜水气单胞菌感染后能很快产生多种抗菌物质,主要的抗菌物质是分子量比较大的物质,这些抗菌物质对部分革兰氏阴性菌和革兰氏阳性菌有抗菌作用。To investigate the antibacterial protein in bullfrog （Rana catesbeiana ） serum, bullfrogs were injected intraperitoneally with Aeromonas hydrophila suspension with a concentration of 1 × 10^7 cells · mE^-1. The antibacterial activities of sera from infected bullfrogs in the period of 7 d post-injection were higher than those of control bullfrogs injected i. p. with sterile physiological saline. The serum antibacterial activity of infected bullfrogs peaked at 3 d post-injection and had a significant difference （ P 〈 0.05 ） to the control counterpart at 3 d and 7 d post-injection. Another group of bullfrogs were injected i. p. with Aeromonas hydrophila, and the sera were collected 3 d post-injection. The sera were separated by Sephadex G-25 gel filtration. The results of OD280 showed that there were 2 main peaks of the fractions collected. The antibacterial activity of serum to Aeromonas hydrophila was determined and the results showed that the first peak contained the main antibacterial protein. The antibacterial range of the antibacterial fractions was determined by using 10 strains as indicator, among them, Escherichia coli, Bacillus subtillis, Pseudomonas aeruginosa were inhibited. Result of SDS-PAGE assay showed high-molecular-weight of the antibacterial protein. The results indicated that bullfrog would produce multi-antibacterial-component soon after being infected by Aeromonas hydrophilia; the major antibacterial protein had high-molecular-weights and inhibited some Gram-positive and Gram-negative bacteria.

关 键 词：牛蛙 嗜水气单胞菌 抗菌蛋白 抗菌活性 

分 类 号：S917[农业科学—水产科学]