恒河猴脊髓半横断损伤后腹角及对侧脑皮质前运动区EGF阳性神经元数的变化  

Changes in Number of EGF Positive Neurons in Ventral Horn and Contralateral Cortex Motor Area of Rhesus after Hemisection Spinal Cord Injury

作　　者：卢永超[1] 齐建国[1] 周雪[1] 王廷华[1] 冯忠堂[2] 

机构地区：[1]四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室,成都610041 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2007年第1期40-44,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨脊髓损伤(SC I)对表皮生长因子(EGF)表达的影响及EGF在脊髓损伤再生修复中的作用。方法18只成年恒河猴被随机分为假手术对照组,脊髓半横断损伤(hSC I)7 d、14 d、1月、2月和3月组,各损伤组于第11胸髓节段切断左侧半脊髓,在相应时间点取材,脊髓取损伤处近、远段5 mm两段,脑取损伤对侧皮质前运动区。制作冰冻切片,采用抗EGF多克隆抗体免疫组织化学SP法染色,观察并计数脊髓腹角及对侧大脑皮质前运动区EGF免疫阳性神经元数的变化。结果恒河猴hSC I后3月内,损伤处脊髓近、远段损伤侧与未损伤侧腹角及对侧皮质前运动区EGF阳性神经元数均较假手术对照组减少(P<0.05),表现为先降低后增加,晚期呈逐渐减少的变化趋势,不同部位EGF阳性神经元数波动不一致。结论恒河猴hSC I后3月内脊髓损伤侧腹角EGF阳性神经元数明显减少;hSC I也会影响未损伤侧EGF的表达;损伤早期,邻近损伤处脊髓腹角及对侧脑皮质前运动区EGF阳性神经元数急剧减少后均有自发回升。Objective To observe the changes in the amount of epidermal growth factor （EGF） immunopositive neurons in ventral horn and contralateral cortex motor area of rhesus following hemisection spinal cord injury （hSCI）. Methods Eighteen adult healthy rhesus were randomly divided into six groups： Sham- operation group; Day 7, Day 14, Month 1, Month 2 and Month 3 hemisection spinal cord injury groups. In the hSCI groups, the monkeys were subjected to left hemisection of T11 spinal cord, and then were put to death at the corresponding time after operation. The rostral part 5 mm proximal to the lesioned point of spinal cord and the caudal part 5 mm distal to the lesioned point were taken from each monkey. The contralateral cortex motor area was taken out, too. Frozen sections were incubated in specific polyclonal anti-EGF antibody; the immunohistochemical SP method was adopted in the study. Results In 3 months after hSCI, the number of EGF immunopositive neurons in the ventral horn of spinal cord near the lesion and in the contralateral cortex motor area of brain decreased as compared with those of the sham-operation group （P〈0. 05）. The number of positive neurons decreased first, then came back, and later after hSCI, decreased again（P〈0. 05）. Besides this, the number of positive neurons varied in different parts at the same time point. Conclusion The EGF immunopositive neurons decreased apparently in the ventral horn of spinal cord near the lesion and in the contralateral cortex motor area in 3 months after hSCL Hemisection spinal cord injury affected the expression of EGF for motor neurons in ventral horn on the lesioned side as well as on the intact side. Early after hSCI the number of positive neurons decreased sharply and then came back spontaneously in the ventral horn of spinal cord near the lesion and in the contralateral cortex of brain.

关 键 词：表皮生长因子 脊髓半横断 免疫组织化学 脊髓 脑 恒河猴 

分 类 号：R651.2[医药卫生—外科学]