鼠疫间接血凝微量检测技术的改进与应用  

Improvement and Application of Indirect Hemagglutination Assay (IHA) of Plague

作　　者：张洪英[1] 洪梅[1] 杨智明[1] 苏丽琼[1] 梁云[1] 宋志忠[1] 董兴齐[1] 

机构地区：[1]云南省地方病防治所,云南大理671000

出　　处：《地方病通报》2007年第1期13-17,共5页Endemic Diseases Bulletin

基　　金：云南省科技攻关资助项目(2002NG17)

摘　　要：目的建立一种简便的鼠疫血清学间接血凝微量法。方法以常规鼠疫间接血凝试验试管法和微量法为基础进行改进。结果改进后的鼠疫间接血凝试验微量法无论在实验室,还是现场应用结果均与试管法一致,但从操作上更为简便、快速,结果判定客观清晰、易于质控,每次检测血清用量仅10μL,试剂耗材节省。结论改进后的鼠疫血清学间接血凝微量法可替代常规鼠疫间接血凝试验试管法和微量法。Objective To establish a simple methods of plague IHA. Methods The methods were improved on base of routine IHA. Results The improving IHA methods were identical with the method of Test Tube at laboratory and spots. It was simple and convenient, rapid, clears good quality control effect. The de- tected sample was only 10μL. Conclusions Improving plague IHA smaller quantity methods may be instead of routine Test Tube methods and micromethod.

关 键 词：鼠疫血清学 间接血凝试验 改进 

分 类 号：R378.61[医药卫生—病原生物学]