苏云金芽孢杆菌HBF-1菌株防治金龟科幼虫的效果评价  

Evaluation of control effect of Bacillus thuringiensis strain HBF-1 against larvae of Scarabaeoidae

作　　者：冯书亮[1] 王容燕[1] 王金耀[1] 杜立新[1] 黄大昉[2] 

机构地区：[1]河北省农林科学院植物保护研究所,保定071000 [2]中国农业科学院生物技术研究所,北京100094

出　　处：《植物保护学报》2006年第4期417-422,共6页Journal of Plant Protection

基　　金：国家自然科学基金(30200184);河北省农林科学院重点项目(A03-1-03-09)

摘　　要：以我国筛选出的对金龟科幼虫具有杀虫活性的HBF-1菌株为供试菌株，采用菌悬液与含有土豆丝的土壤混合饲喂幼虫的方法，测定了HBF-1菌株孢晶粉剂对华北地区主要金龟子幼虫的毒力，其敏感性依次为四纹丽金龟Popillia quadriguttata、黄褐丽金龟Anomala exoleta、蒙古丽全龟A．mongolica、铜绿丽金龟A．corpulenta和苹毛丽金龟Proagopertha lucidula，LC50为3．104、18．987、30．924、34．713和55．995μg／s土。HBF-1菌株悬浮剂防治花生田金龟子幼虫的田间小区试验结果表明，50倍液的虫口减退率为71．4％～75．4％，保果效果为58．7％～72．9％。在花生产量比较试验中，HBF-1菌株悬浮剂50倍液处理区比空白对照区的产量增加363．0—903．0kg／hm^2。HBF-1菌株悬浮剂施入草坪后可免受阳光中紫外线的照射，杀虫活性保持较长时间；经调查，HBF-1制剂在土壤中经过120天后仍保持杀虫活性。To test the toxicity of spore-crystal formulation of HBF-1 specific to larvae of several main scarabaeid species in North China, Bacillus thuringiensis suspensions mixed with sterilized fine soil containing potato pieces were used for feeding the larvae. The LC50 of HBF-1 crystal-spore powder to Popillia quadriguttata, Anomalaexoleta, A. mongolica, A. corpulenta and Proagopertha lucidula were 3. 104, 18. 987, 30.924, 34.713 and 55. 995μg/g soil, respectively. Among them, P. quadriguttata was most sensitive to HBF-1. Plot experiment was conducted to control scarab larvae using HBF-1 SC in peanut field. The number of larvae decreased by 71.4% -75.4% at the 50 times dilution, and its nut-retention rate reached to 58.7% - 72.9%, and the yield of the nut at 50 times dilution increased by 363.0 - 903.0 kg/hm^2 compared to that of the control. After application, the insecticidal activity of HBF-1 in the lawn can persist for a longer period due to avoiding of ultraviolet irradiation, and its activity can keep 120 days after application.

关 键 词：苏云金芽孢杆菌 HBF-1菌株 金龟科 花生 草坪 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]