鸡FASN基因2个位点的多样性及其与体重、脂肪沉积性状的相关性  

Genetic Diversity of Two Sites of FASN Gene and Its Association with Growth and Fat Deposition in Chickens

作　　者：欧阳建华[1] 谢亮[2] 刘杰[1] 骆毅媛[1] 梁章敏[1] 聂庆华[1] 张细权[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]江西农业大学动物科技学院,南昌330045

出　　处：《畜牧兽医学报》2007年第1期25-30,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"高技术发展计划(2002AA242102)

摘　　要：研究了脂肪酸合成酶(Fatty acid synthase,FASN)基因最后1个内含子2个突变的多样性,并运用资源参考家系研究了这2个突变对鸡体重、脂肪沉积性状的影响,结果表明:(1)红色原鸡HaeⅢ-PCR-RFLP位点B基因频率为最高(0.85),泰和鸡及余干乌黑鸡为最低(0.21),品种间差异较大,但未发现肉用与蛋用鸡种间的差异;在PstI-PCR-RFLP位点上,A基因频率在崇仁麻鸡中表现为最高(0.5),在裸颈鸡为最低(0.19),品种间差异较小。(2)HaeⅢ-PCR-RFLP位点表现为CC型个体体重最小,大部分阶段有显著性差异(P<0.05);PstI-PCR-RFLP位点表现为差异不显著。2位点合并基因型中,以BC/GG体重为最高,大部分阶段有显著性差异(P<0.05)。(3)HaeⅢ-PCR-RFLP位点表现为BB型比其它基因型有更强的脂肪沉积能力(P<0.05),而PstI-PCR-RFLP位点不同基因型间未表现出脂肪沉积能力的差异,合并基因型中以BB/GG脂肪沉积能力最强(P<0.05)。(4)2位点在脂肪沉积和体重上均表现一定的互作效应。In this study, two SNPs of chicken FASN gene were chosen to analyze genetic diversity and their associations with chicken growth and carcass traits by using a F2 designed resource population. Results showed that.. （1） In the PCR-RFLP site of Hae Ⅲ , allelic frequencies differed much among different chicken breeds, as B frequencies are the highest in Red Jungle Fowl （0.85）, and the lowest in Taihe and Yugan Black （both 0.21）. In the PCR-RFLP site of Pst Ⅰ , A frequencies are the highest in Chongren Partridge （0. 5）, and the lowest in Naked Neck chicken （0. 19）, and the difference of gene frequencies was small among chicken breeds. （2） In Hae Ⅲ site, it was significantly associated with most growth traits, individuals with CC genotype had the lowest body weight; No significantly difference was found at Pst Ⅰ site; When considering combined genotypes of the two SNPs, individuals with （BC/GG） genotype had the highest body weight. （3） In HaeⅢ site, individuals with BB genotype had the higher fat deposition caoacity than that of others, however no significant differences in fat deposition were observed among different genotypes in Pst Ⅰ site And, when considering combined genotypes of the two SNPs, individuals with （BC/GG） genotype had the highest fat deposition capacity. （4） There were certain interactional effects within the two SNPs including bodyweight and fat deposition traits.

关 键 词：鸡 FASN 遗传多样性 体重 脂肪沉积性状 

分 类 号：S831.2[农业科学—畜牧学]