GNRHRI、GF-1基因对文昌鸡繁殖性状的遗传效应分析  

Genetic Effects of GNRHR and IGF-1 Genes on the Reproductive Traits in Wenchang Chicken

作　　者：吴旭[1] 王金玉[1] 严美姣[1] 李慧芳[2] 陈宽维[2] 汤青萍[2] 朱文奇[1] 俞亚波[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]中国农业科学院家禽研究所,扬州225003

出　　处：《畜牧兽医学报》2007年第1期31-35,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技基础条件平台(2004DKA30450-04);国家高技术研究发展计划(2001AA243081)

摘　　要：以控制文昌鸡繁殖性状的促性腺激素释放激素受体基因(GNRHR)和胰岛素样生长因子-1基因(IGF-1)为候选基因,采用PCR-RFLP技术检测GNRHR基因和IGF-1基因在文昌鸡中的单核苷酸多态性(SNP),同时对这2个基因与文昌鸡繁殖性状的相关性进行了研究。结果表明,GNRHR基因内含子1的537 bp位置有C→T碱基的变异,在文昌鸡中检测到AA、Aa、aa 3种基因型,A等位基因的频率为0.69,a等位基因的频率为0.31;在IGF-1基因5′非翻译区(5′UTR)发现C→T碱基的变异,改变了限制性内切酶PstI识别位点,经PCR-RFLP分析,在文昌鸡中检测到BB、Bb、bb 3种基因型,B等位基因的频率为0.53,b等位基因的频率为0.47。对其基因型与所检测个体相应的繁殖性状采用GLM分析进行遗传效应研究,结果表明,GNRHR基因对300日龄产蛋数、400日龄产蛋数有显著影响(显性作用),IGF-1基因对300日龄产蛋数有显著影响(加性作用)。因此,推测GNRHR基因和IGF-1基因可能是影响鸡繁殖性状的主效基因或与主效基因紧密连锁的标记基因,并且能够在分子标记辅助选择中用于对鸡繁殖性状的遗传改良和固定。Gonadotropin-releasing hormone receptor （GNRHR） gene and insulin-like growh factor 1 （IGF-1） gene which control the reproductive traits were studied as candidate genes on the Wenchang chicken（Chinese indigenous breed）in the present study. The single nucleotide polymorphism of GNRHR and IGF-1 was detected for the association with the total egg production （NE） ,average days of continual egg-laying （ADCE） and number of double-yolked eggs （DYE） in Wenchang chicken. PCR-RFLP method was used for identification of genotypes. The frequency of restriction enzyme A/a alleles in the population was for GNRHR 0. 69 （Bpu1102IA） and 0.31 （Bpu1102I a） and for IGF-10. 53 （PstI B） and 0.47 （PstI b）. χ^2 analysis of the two genes indicated that the frequencies of GNRHR, IGF-1 fit Hardy-Weinberg equilibrium. The result showed that GNRHR Bpu1102I Aa genotype had greater number of eggs （300d） at 87. 91 compared to 81.20 for Bpull02I AA （P〈0. 01）. The GNRHR Bpu1102I Aa genotype had greater number of eggs （400 d） at 135.47 compared to 125.47 for Bpu1102I AA （P〈0. 01）. The IGF-1 PstI bb genotype had greater number of eggs （300 d） at 89.03 compared to 82.61 for Pst Ⅰ BB （P〈 0.05） and Pst Ⅰ bb genotype also had greater number of eggs （400 d） at 137.84 compared to 127.82 for Pst Ⅰ BB （P〈0. 05）. The IGF-1 Pst Ⅰ bb genotype had greater ADCE at 3.38 compared to 2.96.2.78 for Pst Ⅰ BB and Bb （P〈0. 05） . Two significant effects of genes＇ marker were found： GNRHR of dominant effect on number of eggs and IGF-1 of additive effect on that. The current research supports the effects of GNRHR and IGF-1 genes on reproductive traits of chickens.

关 键 词：鸡 GNRHR基因 IGF-1基因 繁殖 SNP 

分 类 号：S831.2[农业科学—畜牧学]