婴儿巨细胞病毒性肝炎炎性细胞因子水平及其与肝功能损害的关系  

Serum cytokine levels and their correlation with ALT in infants with cytomegalovirus hepatitis

作　　者：熊菀[1] 刘瑜[2] 陈琼[2] 吴扬[3] 

机构地区：[1]重庆医科大学附属儿童医院感染消化科,400014 [2]重庆医科大学附属儿童医院儿科研究所,400014 [3]重庆医科大学附属儿童医院检验科,400014

出　　处：《检验医学与临床》2007年第1期2-3,共2页Laboratory Medicine and Clinic

摘　　要：目的了解婴儿巨细胞病毒(CMV)肝炎患儿肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)/白细胞介素4(IL-4)及丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平比值,探讨其在炎症过程中的作用及相关关系。方法应用酶联免疫吸附试验(ELISA)法检测TNF-α、IFN-γ/IL-4水平,用全自动生化分析仪检测ALT和AST水平。共分析了CMV肝炎30例,正常对照组30例。结果CMV肝炎组TNF-α、IFN-γ/IL-4、ALT、AST值分别为448.07±122.76ng/L,2.99±1.35,95.24±8.52U/L,138.59±11.57U/L,TNF-α、ALT、AST均高于正常对照组,IFN-γ/IL-4比值低于对照组。相关分析发现,肝炎组TNF-α与ALT呈正相关(r=0.76,P<0.01)。结论CMV肝炎患儿存在细胞因子分泌紊乱,表现在TNF-α水平增高,与肝功能损害程度密切相关;IFN-γ/IL-4比值变化推测存在Th1/Th2平衡失调和Th2功能异常增强情况。Objective To investigate the serum cytokine levels and to explore their correlation with ALT in infants with cytomegalovirus hepatitis. Methods ELISA method and automatic analysis machine were used to determine TNF-α, ALT,AST and IFN-γ/IL-4 in 30 cases of CMV- infected hepatitis and 30 infants as control group. Reshits The levels of TNF-α,IFN-γ/IL-4,ALT and AST in CMV infected hepatitis group were 448.07 ± 122.76 ng/ L,2. 99±1.35,95. 24±8. 52 U/L,138.59±11.57 U/L. In CMV- infected hepatitis group all increased as compared with the normal group . But the IFN-γ/IL-4 ratio （2. 99 ± 1.35） decreased as compared with the normal group. There was a positive correlation between TNF-α and ALT（r=0.34,P〉0.05）. Conclusion There is abnormal cyto kines secretion in infants with CMV- infected hepatitis. Elevated TNF-α level is closely related with the liver lesion extent ,and the change of IFN-γ/IL-4 ratio is the index of the Th1/Th2 imbalance.

关 键 词：婴儿巨细胞病毒性肝炎 细胞因子 丙氨酸氨基转移酶 

分 类 号：R725.7[医药卫生—儿科]