异福酰胺片的近红外光谱法测定  

Determination of Rifampicin,Isoniazid and Pyrazinamide Tablets by NIRS

作　　者：蒋朝军[1] 逯家辉[1] 申斯乐[1] 郭伟良[1] 滕利荣[1] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《中国医药工业杂志》2007年第1期32-35,共4页Chinese Journal of Pharmaceuticals

基　　金：吉林省科技发展基金(20020503-2)

摘　　要：采用近红外光谱法快速定量分析异福酰胺片。按处方配制41个实验室样品,并收集10批市售产品,采集近红外光谱。分别对光谱进行卷积平滑、快速傅里叶变换、一阶导数和二阶导数预处理,采用偏最小二乘回归法建立样品中主药含量的定量分析模型。模型对15个预测集样品进行预测结果为:利福平、异烟肼、吡嗪酰胺模型的交互验证均方差RMSECV分别为0.00287、0.00216和0.00435,平均回收率分别为100.7%、100.3%和99.8%,RSD分别为1.5%、2.1%和0.7%。A near infrared reflectance spectroscopy （NIRS） was used to the determination of the content of rifampicin, isoniazid and pyrazinamide tablets. Forty one samples were prepared in the laboratory and ten commercial samples were collected. The NIR spectra were collected and then be pretreated by Savitzky-Golay, fast Fourier transform, first order derivative and second order derivative. The partial least squares regression algorithm was used to build the quantitative analysis model. Results of 15 predicted set samples were as follows： the root mean square error of crossvalidation of the models of rifampicin, isoniazid and pyrazinamide were 0.00287, 0.00216 and 0.00435, respectively. The average recoveries were100.7%, 100.3% and 99.8%, with the RSDs of 1.5%, 2.1% and 0.7%, respectively.

关 键 词：利福平 异烟肼 吡嗪酰胺 近红外光谱法 偏最小二乘 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.33[化学工程—制药化工]