花生白藜芦醇合酶基因转化单子叶植物表达载体的构建  

Construction of the Monocotyledon Expression Vector for the Transforming of Resveratrol Synthase Gene

作　　者：许玉芬[1] 叶冰莹[1] 陈由强[1] 王冰梅[1] 黄庆煌[1] 林志楷[1] 陈如凯[2] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2007年第1期83-86,95,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30371177);福建省自然科学基金资助项目(B0310004);卫生部基金资助项目(JA05225/WKJ2);福建省科技厅基金资助项目(2005N037)

摘　　要：构建了花生白藜芦醇合酶基因(RS)转化单子叶植物的表达载体,该表达载体含有ub i启动子和内含子,能启动该基因在单子叶植物中高效地表达.通过PCR反应扩增出目的片段,连接到克隆载体Pub i35s上,切下含ub i和RS约3 000 bp的片段连接到植物表达载体pCAM B IA-1 380上.经PCR和酶切检测,结果与预期相同,经测序确定插入片段读码框正确.该表达载体可用于单子叶植物高效的表达.A monocotyledon expression vector was constructed for transforming Resveratrol Synthase （RS） gene. This vector contains ubi promoter and intron which can drive high expression of the gene in monocotyledon . PCR was used to amplify the RS genc. A new cloning vector was then constructed by inserting the amplified RS gene into Pubi35s. The monocotyledon expression vector was constructed by inserting the ubi promoter and RS gene （about 3 000 bp） into pCAMBIA-1 380, which cutted from the new cloning vector above. PCR and restriction enzyme digesting analysis showed that the inserting DNA fragment was the size of expecting. Furthermore , the sequencing showed that the insert DNA fragment was not out of frame. The expression vector can be used for the high expression in monocotyledon.

关 键 词：花生白藜芦醇合酶基因 UBI 单子叶植物 表达载体 构建 

分 类 号：Q784[生物学—分子生物学]