龙眼梅PGIP基因的克隆及序列分析  

Cloning and sequencing of PGIP Gene from Longyanmei,a cultivar of Prunus mume

作　　者：王家福[1] 朱春林[1] 陈桂信[2] 潘东明[2] 潘才博[1] 叶露莹[2] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002 [2]福建农林大学园艺学院,福州350002

出　　处：《果树学报》2007年第1期55-58,共4页Journal of Fruit Science

基　　金：国家948项目(991029);福建省科技项目(2001I008)。

摘　　要：利用逆转录-聚合酶链式反应(RT-PCR)得到在龙眼梅(PrunusmumeSiebetZucc.Longyan)嫩芽中特异性表达的PGIP基因。DNA序列分析表明,所获得龙眼梅的PGIPcDNA的序列全长为1045bp,该序列与已发表的PGIP基因有很高的同源性。The PGIP gene in Prunus mume Sieb et Zucc. Longyan, which was specifically expressed in tender bud, was ohtained by reverse transcriptase-polymerase chain reaction （RT-PCR）. Destination segments amplified by PCR were reclaimed , connected , transformed and sequence analysed .The results showed that the PGIP cDNA of Prunus mume Sieb et Zucc. Longyan was 1045 bp. The sequence had high identities with that of PGIP gene which had been cloned.

关 键 词：龙眼梅 PGIP基因 克隆 

分 类 号：S662.4[农业科学—果树学]