免疫磁珠负性分离纯化小鼠脾脏CD8^+ T细胞  

MACS negative separation of CD8^+ T cells from murine spleen CD8^+ T cells

作　　者：杨曌[1] 傅晓岚[1] 尚小云[1] 吴玉章[1] 赵建平[1] 王莉[1] 

机构地区：[1]第三军医大学基础医学部全军免疫研究所,重庆400038

出　　处：《免疫学杂志》2007年第1期91-93,共3页Immunological Journal

基　　金：国家自然科学基金资助项目(30400437;30371329)

摘　　要：目的探讨小鼠脾脏CD8^+ T细胞的免疫磁珠负性分选方法,并对分选后所得细胞进行纯度、活力及功能检测。方法以免疫磁珠负性分选法从小鼠脾脏细胞中分离CD8^+ T细胞,流式细胞术检测所得细胞的纯度,台盼蓝检测细胞活力并用ConA刺激检测增殖能力。结果经过流式细胞仪测定免疫磁珠负性分选后的小鼠脾脏CD8^+ T细胞纯度达到(91.6±3.6)%,台盼兰染色细胞活力为(94.9±3.2)%,ConA刺激72h后有(56.3±1.7)%的细胞增殖。结论免疫磁珠负性分选法能够分选出高纯度的CD8^+ T细胞,并且不影响分选靶细胞的细胞活力和功能。Objective To assess the separation efficiency of magnetic-activated eel sorting （MACS） in the puxification of CD8^＋ T cells from murine spleen. Methods CD8^＋ T cells were isolated from murine spleen by MACS. Fluorescence-activated cell sorter was employed to determine the purity of CD^8 ＋ T cells before and after the separation procedure. The viability and proliferation of the stored CD8^＋ T cell were evaluated by trypan blue staining and ConA stimulation, respectively. Results The purity of CD8^ ＋ T cells reached （91.6± 3.6）% after the purification with cell viability of （ 94.9 ± 3.2） % and proliferation of （56.3 ± 1.7） %. ,Conclusion CD8^ ＋ T cells of high purity can be obtained from murine spleen using MACS without impairing the viability of the cells.

关 键 词：CD8^+T细胞 免疫磁珠负性分选 

分 类 号：R392.14[医药卫生—免疫学]