荧光极性探针在腐殖酸和芘相互作用研究中的应用  

Study on the Interaction Between Humic Acid and Pyrene by Polarity Probe

作　　者：李毓骐[1] 朱亚先[1] 鹿贞彬[2] 彭景吓[3] 王萍[3] 蔡宗群[3] 肖翔[3] 张勇[3] 

机构地区：[1]厦门大学化学化工学院化学系,福建厦门361005 [2]华侨大学环境保护设计研究所,福建泉州362021 [3]近海海洋环境科学国家重点实验室(厦门大学)厦门大学环境科学研究中心,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2007年第1期59-62,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(20577037)资助

摘　　要：芘(Pyrene,Py)的荧光发射光谱的第一振动带和第三振动带强度比值(I1/I3)能够反映其所处微环境的极性,因此运用Py荧光探针法考察了不同化学条件对腐植酸(Humic Acid,HA)假胶束体系微环境性质的影响,以及对HA和Py之间相互作用的影响.结果表明:HA浓度、pH值、盐度、溶剂等化学条件的改变,均会引起Py的I1/I3的变化,因为条件的变化使HA存在形态发生变化,从而导致Py所处为环境极性的变化.另外,重金属离子浓度的变化也可以影响HA和Py之间相互作用的影响,随着重金属离子浓度的增大I1/I3值遵循三段式变化趋势.这些现象均表明HA和PAHs之间的作用以非极性相互作用为主.In the fluorescence spectra of Pyrene（Py）,the ratio of fluorescence emission intensitise corresponding to the first and third peaks（I1/I3 ） was depended on the polarity of the microenvironment where Py located. The effects of the different chemical conditions on the microenvironment of Humic acid （HA） and the interactions between HA and Py were investigated by polarity probe method. The variation of the I1/I3 value of Py was studied in the different pH values, as well as in the different concentrations of HA, sodium chloride and heavy metal ions. The results showed that the changes of chemical condition resulted in the variations of the I1/I3 value of Py. Those sets of effects were explained in terms of conformational adjustments of HA. Furthermore,different types of heavy metal ions had different effects on the interactions between HA and the PAHs. To describe the complicated effects of heavy metal ions,we use the three-stage variation model that encompasses decreasing and increasing trends and plateaus. The mechanisms were explained in the variation of HA structure configuration, HA aggregation and the salting-out effect. All of the results proved that the interactions between HA and Pyrene Py were nonpolar.

关 键 词：腐植酸 荧光探针 芘 

分 类 号：O657.3[理学—分析化学]