黑龙江省东部边境口岸莱姆病生物媒介调查及病原分型研究  

Investigation on the vectors of Borrelia burgdorferi and on the identification of the isolates along China- Russia borter in Eastern Heilongjiang province,China

作　　者：何浩[1] 郝琴[2] 呼满霞[1] 候学霞[2] 范东晖[1] 耿震[2] 张健[1] 张少华[1] 郑超[1] 吴永生[1] 王传松[1] 崔文富[1] 万康林[2] 杨丽炜 

机构地区：[1]黑龙江绥芬河出入境检验检疫局,157300 [2]中国疾病预防控制中心传染病预防控制所 传染病预防控制国家重点实验室 [3]黑龙江出入境检验检疫局

出　　处：《中华流行病学杂志》2007年第1期70-73,共4页Chinese Journal of Epidemiology

基　　金：国家“十五”科技攻关项目（2001BA705807）;绥芬河出人境检验检疫局、中国疾病预防控制中心传染病预防控制所和黑龙江出入境检验检疫局对本文有同等贡献.在本课题研究过程中得到东宁出入境检验检疫局、密山出入境检验检疫局、虎林出入境检验检疫局、饶河出入境检验检疫局、抚远出入境检验检疫局、同江出入境检验检疫局的大力支持和协助,在此衷心感谢.

摘　　要：目的调查黑龙江省东部中俄边境口岸蜱的种类、分布、携带莱姆病螺旋体情况及病原基因型。方法捕蜱采用布旗法；蜱带莱姆病螺旋体率采用直接荧光法检查；病原体分离培养采用BSKII培养基，对分离到的螺旋体用单克隆抗体（H5332、H6831、H9724）、SDS-PAGE蛋白图谱分析、PCR-RFLP分析以及基因测序等方法进行表型和基因分型研究。结果黑龙江省东部7个中俄边境口岸蜱的种类为1科3属4种，即硬蜱科硬蜱属全沟硬蜱，革蜱属森林革蜱，血蜱属嗜群血蜱和日本血蜱。不同生态环境中蜱的分布不同，各口岸优势蜱种不同。全沟硬蜱带莱姆病螺旋体率较高，平均为31．4％，森林革蜱和嗜群血蜱的带菌率较低，平均为2．2％和3．8％，日本血蜱中未检出阳性者。首次在中俄边境口岸——东宁、同江采集的全沟硬蜱中分离到伯氏疏螺旋体并鉴定为B．garinii基因型。结论黑龙江省东部边境口岸为莱姆病的自然疫源地。Objective To explore the fact that the east border of Heilongjiang province had been a lyme disease natural focus, we investigated the species and distribution of ticks and isolated bacteria from ticks and identified genomic species of Borrelia burdorferi sensu lato, This study provided evidence for prevention and control of lyme disease. Methods Ticks were caught by flagging method arid Direct immunofluorescenee method was used to detect the rate of bacteria borne by the tick, BSK Ⅱ culture medium was used to isolate the agent and Specific McAbs were used to identify the bacteria. SDS-PAGE protein profile and PCR-RFLP method were also used to identify the species of Spirochetes. Results Ticks, collected from China-Russia border of east Heilongjiang province were classified including Ixodes persulcatus Schulze, Dermacentor sivarum Olener , Haemaphysalis concinna Kock,and Haemaphysalis japonica Koek. We found that the distributon of ticks was different under different circumstances and the predominant species were also different in different ports. The rate of bacteria borne by Ixodes persulaatus Schulze was 31.4 % ,by Dermacentor sivarum Olener and Haemaphysalis concinna Koek were 2.2 % and 3.8 %, respectively. However, it was negative for Haemaphysalis japonica Koek, Spirochetes isolated from Ixodes persulcatus Schulze were collected from Dongning and Tongjiang while Genomie species of Spirochetes, isolated from ticks of the border belonged to B. garinii. Conclusion All the results showed that the east border of Heilongjiang province was the natural focus of lyme disease.

关 键 词：莱姆病 蜱 伯氏疏螺旋体 基因分型 

分 类 号：R686[医药卫生—骨科学]