交链孢菌中一种酸性糖蛋白的鉴定及功能分析  

Purification and Characterization of a Glycoprotein from Alternaria tenaissime

作　　者：郭广君[1] 邱德文[2] 吕素芳[1] 王荣富[1] 刘峥[2] 杨秀芬[2] 赵明治[1] 董健伸[2] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]中国农业科学院植物保护研究所,北京100081

出　　处：《科学技术与工程》2007年第3期281-284,共4页Science Technology and Engineering

基　　金：国家重点基础研究发展计划(973计划)(2003CB114204);国家高技术研究发展计划(863计划)资助(NO.2003AA241130)资助

摘　　要：采用液氮研磨、离心、阴离子交换柱HitrapTMDEAE FF、分子筛柱Hitrap Superdex75/300等一系列分离纯化方法对细极链格胞(Aternaria tenuissima)水溶性酸性糖蛋白激发子进行了纯化。经SDS-PAGE、Native-PAGE检测,蛋白分子量约为66KDa;经全蛋白银染和糖蛋白染色试剂盒对其鉴定为糖蛋白。经蒽酮-硫酸法测定,糖与蛋白比例为1.75。经β-消去反应判定糖蛋白的连接类型为O-连接。生物活性测定表明,该蛋白可促进小麦种子的萌发和幼苗的生长。C A acidic glycoprotein from Alternaria tenuissima, had been isolated and purified by anion-exchange FF and molecular sieve chromatography on Hitrap Superdex 75/300. The glycoprotein was proved to have the molecular weight of about 66 kD by Native-PAGE and SDS-PAGE. The glycoprotein was identified that the carbohydrate and protein contents were in a ratio of 1.75 by GelCode Glycoprotein Staining Kit（ Pierce accompany ）, antrone-colorimetric assay. The type of glycocidic bond was judged by β-elimination reaction oxygen juncture. Bioassay detection indicated that the glycoprotein could increase the sprouting rate and promote the growth of wheat seeds.

关 键 词：细极链格胞 糖蛋白 β-消去反应 

分 类 号：S184[农业科学—农业基础科学]