硫化氢缓解自发性高血压大鼠主动脉重构中胶原堆积  

Hydrogen sulfide alleviate accumulation of collagen in aorta of spontaneous hypertensive rats

作　　者：张春雨[1] 杜军保[1] 闫辉[1] 唐朝枢[2] 

机构地区：[1]北京大学第一医院儿科 [2]北京大学第一医院心血管研究所

出　　处：《基础医学与临床》2006年第10期1052-1055,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30271373);教育部高等学校博士学科点专项基金(20020001063);国家重点基础研究发展规划项目(G2000056905);国家杰出青年科学基金(30425010)

摘　　要：目的以自发性高血压(SHR)大鼠为对象,研究硫化氢(H2S)对血管中胶原堆积的影响。方法SHR大鼠17只,随机分为SHR组(n=6),SHR+H2S(n=6)组和SHR+PPG组(n=5)。WKY大鼠5只为对照组(n=5)。SHR+H2S组每日腹腔注射0.25 mL H2S供体NaHS(56μmol/kg),SHR+PPG组每日腹腔注射0.25 mL PPG(37.5 mg/Kg),连续用药5周。WKY对照组及SHR组注射等量注射用水。连续混合喂养5周后测定动脉血压;以胸主动脉组织为对象,用酶促反应法测定CSE活性;用高温裂解法测定羟脯氨酸含量。用酶联免疫法(ELISA)测定Ⅰ型胶原含量。用免疫组化法检测转化生长因子β3(TGF-β3)表达。结果与WKY对照组相比,SHR组大鼠血压升高52%(P<0.01);CSE酶活性降低55%(P<0.01);羟脯氨酸含量增加87%(P<0.01);Ⅰ型胶原含量增加28%;TGF-β3表达增加10%(P<0.05)。给予H2S后缓解了上述指标的变化。结论给SHR大鼠补充外源性H2S能够降低动脉血压,而且使主动脉组织中羟脯氨酸和Ⅰ型胶原含量减少,从而改善SHR大鼠血管中胶原的堆积。Objective To study the regulatory effect of hydrogen sulfide （H2S） on the accumulation of collagen in thoracic aorta of spontaneous hypertensive rats （SHR）. Methods Seventeen male SHR rats were randomly divided into SHR group （n =6）, SHR ＋ H2S group （n =6）, SHR ＋PPG group （n =5）. Five WKY rats were included as WKY group （n =5）. NariS, which is H2S donator, was injected peritoneally into rats of SHR ＋ H2S group with a dosage of 56μmol/kg everyday for 5 weeks. For rats in SHR ＋ PPG group, D, L-propargylglycine （ PPG）, which is CSE inhibitor, was peritoneally injected with a dosage of 37.5 mg/kg for 5 weeks. The other two groups of rats were injected with the same volume of sterile water. At the age of 9 weeks, the arterial blood pressure was measured by tail capacity method. Using the thoracic aorta as study object, the enzymatic activity of cystathionine T-lyase （CSE） was determined by enzyme facilitating reaction; the hydroxyproline quantification was measured by hyperthennia splitting method; the amount of collagen type Ⅰ was determined by ELISA method ; the expression of transforming growth factor β3 （TGF-β3） was detected by immunohistochemistry method. Results For rats in SHR group, the blood pressure increased by 52% ; the CSE activity decreased by 55% （ P 〈 0.01 ） ; the content of hydroxyproline increased by 87% （P 〈0. 01 ） ; the amount of collagen type Ⅰ increased by 28% ; the expression of TGF-β3 in the aortic tissue of SHR group increased by 10% （P 〈 0. 05） as compared with rats in WKY group. Supplying with exogenous H2S alleviate the changes of these parameters above. Conclusion Supplying exogenous H2S to SHR rats could decrease the blood pressure and reduce the content of hydroxyproline and collagen type Ⅰ in the vessels, therefore, lessen the accumulation of collagen in the thoracic aorta of SHR.

关 键 词：硫化氢 高血压 主动脉 重构 胶原 

分 类 号：R544.1[医药卫生—心血管疾病]