间接免疫荧光法快速鉴别白念珠菌和都柏林念珠菌的初步研究  

Identification of Candida albicans and Candida dubliniensis by indirect immunofluorescence

作　　者：孙仁美[1] 汤桂丽[1] 王鲁[2] 

机构地区：[1]解放军324医院,重庆400020 [2]重庆市第一人民医院皮肤科,重庆400011

出　　处：《中国真菌学杂志》2006年第6期327-330,共4页Chinese Journal of Mycology

摘　　要：目的用一种新制备的单克隆抗体MAb03.2C1-C2鉴别生物学形态相近的白念珠菌和都柏林念珠菌。方法用小鼠体内诱导法制备抗白念珠菌芽管胞壁外膜单克隆抗体MAb03.2C1-C2。用不完全RPMI1640培养液、L-DMEM、H-DMEM、完全1640液、小牛血清诱导白念珠菌和都柏林念珠菌芽管及菌丝形成,间接免疫荧光(IIF)方法检测都柏林念珠菌芽管或菌丝表面有无可与该单抗相结合的成分。收集临床口腔念珠菌病标本涂片,直接做IIF试验。结果用不完全RP-MI1640培养液37℃,6h可同时最高效率地诱导白念珠菌和都柏林念珠菌芽管或菌丝形成。单抗MAb03.2C1-C2仅与白念珠菌芽管或菌丝特异性地结合,与都柏林念珠菌的孢子和菌丝不能结合。结论单抗MAb03.2C1-C2可用于白念珠菌和都柏林念珠菌实验室的速鉴别。Objective The monoclonal antibody（MAb） 03.2C1-C2 against germ tube of Candida albicans （ C. albicans ） was used to identify the two closely related species C. albicans and C. dubliniensis. Methods MAb03.2Cl C2 was prepared via hybridoma technique. Different liquid cultures were used to induce the formation of germ tube or mycelium. Indirect immunofluorescence （IIF） test was used to detect whether there were antigens on the surface of the Candida dubliniensis mycelium. Eightyone smear specimens of oral candidiasis was deteceted by IIF test to be C. albicans. Results The germ tube or mycelium could be induced with the highest efficiency after 6 hours in the 1640 culture under 37℃. MAb03.2Cl -C2 was shown by IIF to be specific to the surface of the germ tube of C. albicans . No other Candida species was observed, including spore or mycelium, to react with MAb03.2Cl -C2. Conclusion This anti-C, albicans cell wall surface-specific monoclonal antibody may be a good candidate in test for the rapid differentiation of the two closely related species C. albicans and C. dubliniensis.

关 键 词：白念珠菌 都柏林念珠菌 特异性 单克隆抗体 

分 类 号：R379.4[医药卫生—病原生物学]