培养条件对HBsAg转基因人参细胞的生长及表达量的影响  

Influence of Culture Condition on Growth of HBsAg-transgenic Ginseng Cells and Expression of HBsAg

作　　者：李娟[1] 盛维瑾[2] 刘大为 刘丹[1] 刘晓宇[1] 高正伦[2] 富锐丽[1] 高平[1] 徐彦红 盛军[1] 

机构地区：[1]长春生物制品研究所天然药物研究室,长春130062 [2]吉林大学,长春130023 [3]中国生物技术集团公司,北京100037 [4]长春长生基因药业股份有限公司,长春130012

出　　处：《中国生物制品学杂志》2007年第1期40-42,46,共4页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(No.0030405)资助.

摘　　要：目的研究培养条件对HBsAg转基因人参细胞生长及HBsAg表达量的影响。方法通过单因子实验,分别考察碳源、氮源、有机成分对HBsAg转基因人参细胞生长和HBsAg表达量的影响,并通过不同组合实验,探索氮源浓度、有机成分和起始pH的最佳组合。结果在3%蔗糖、10mmol/L氮源、0·25%乳清蛋白和起始pH6·2的情况下,细胞生长量略有增加,HBsAg的表达量增加1倍。结论通过改善培养条件,可以提高HBsAg转基因人参细胞的生长速度和抗原表达量。Objective To explore the optimal condition for culture of HBsAg-transgenic ginseng cells and expression of HBsAg. Methods Observe the growth of HBsAg-transgenic ginseng cells and expression of HBsAg in media with various carbon sources, nitrogen sources and organic components at various starting pH values. Results The growth of HBsAg-transgenic ginseng cells cultured in the medium containing 3% sucrose,0. 25% lactoalbumin and 10 mmol/L of inorganic nitrogen at a starting pH value of 6. 2 increased slightly,and the expression of HBsAg increased doubly. Conclusion Both the growth of HBsAg-transgenic ginseng cells and the expression of HBsAg were promoted by improving the culture condition.

关 键 词：乙型肝炎表面抗原 人参细胞 生长 表达 

分 类 号：R392-33[医药卫生—免疫学]