黄芪多糖对2型糖尿病KKAy小鼠早期肾脏病理改变的影响  

Effects of Astragalus Polysaccharide on Renal Pathological Changes in the Early Period of Type 2 Diabetic KKAy Mice Model

作　　者：郭鹏[1] 欧阳静萍[1] 毛先晴[1] 张敬芳[1] 

机构地区：[1]武汉大学医学院病理生理学教研室/湖北省过敏及免疫相关疾病重点实验室,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2007年第1期74-76,92,F0003,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30370673)

摘　　要：目的:探讨黄芪多糖(APS)对2型糖尿病小鼠(KKAy)早期肾脏病理改变的影响。方法:雌性KKAy小鼠和C57BL/6J小鼠随机分为:糖尿病组(DM,n=8)、糖尿病黄芪多糖治疗组(DA,n=8)、正常对照组(C,n=10)和黄芪多糖对照组(CA,n=10)。定期监测体重、血糖、血胰岛素水平、计算HOMA-IR指数;观察肾小球病理变化。结果:KKAy鼠较C57BL/6J鼠体重、血糖、血胰岛素及HOMA-IR均显著升高(P<0.01)。DM组小鼠肾小球面积较C组增大,系膜基质增加、肾小管管腔扩大、管壁变薄、肾小球基底膜(GBM)增厚、蛋白管型明显。经APS治疗后,以上指标均明显改善(P<0.01)。结论:APS可降低血糖,增强机体对胰岛素敏感性,对2型糖尿病小鼠早期肾脏病理改变具有良好的治疗作用。Objective： To investigate the effects of Astragalus Polysaccharide （APS） on renal pathological changes in the early period of type 2 diabetic KKAy mice model. Methods： Female KKAy and C57BL/6J mice were assigned into four groups randomly： diabetes （DM） group （n=8） ; diabetes with APS therapy （DA） group （n=8） ; control （C） group （n=10） and APS control （CA） group （n=10）. Body weight, blood glucose was detected. Insulin sensitivity was analyzed by blood insulin level and HOMA IR index was calculated. Early renal pathological variation was presented by histochemical staining and TEM. Results： After APS therapy for 8 weeks, the level of blood glucose, blood insulin, body weight and HOMA IR index was decreased in DA group （P〈0.01, vs DM group）. In DM group mice, renal glomerulus area was magnified, mesenterium basilaris substantia was increased, urinary cast lumina was enlarged, its vessel wall was thinner, GBM was thickening and obvious protein casts were observed compared with those of C group. APS treatment can alleviate these pathological changes. Conclusion： The results indicate that APS can ameliorate the renal pathological variation in the early period, by its effects of insulin-sensitizing and hypoglycemic activity.

关 键 词：黄芪多糖 2型糖尿病 KKAY小鼠 肾脏病理改变 

分 类 号：R587.1[医药卫生—内分泌]