华南鳞盖蕨组织培养与假植技术研究  

Studies on Tissue Culture and Primary Nursery Transplanting of Cephalotaxus

作　　者：郭文杰[1] 蔡宣梅[1] 方少忠[1] 林真[1] 鲁雪华[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福州350003

出　　处：《中国农学通报》2007年第1期50-52,共3页Chinese Agricultural Science Bulletin

基　　金：福建省科技厅重点项目"一种替代三尖杉的草本植物的组织培养和药用成分分析研究(2002Y024-2)。

摘　　要：试验进行了华南鳞盖蕨孢子果的萌发试验,并利用幼嫩茎段进行诱导愈伤组织;愈伤组织的诱导与增殖培养基为MS+6-BA2mg/L+NAA0.2mg/L;其芽分化最佳激素组合为MS+6-BA0.4mg/L;生根培养中MS基本培养基比1/2MS效果好,MS基本培养基不论附加生长素IBA、NAA或IBA+NAA,均能有效地诱导植株生根,生根率高;假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙,具有通气、保水、营养等优点,假植成活率高,苗势健壮。Studies on Tissue Culture and Primary Nursery G The germination test of Sporocarps of Microlepia hancei prantl had been done, the callus can be induced from the delicate stem of Microlepia hancei prantl. The inducement and proliferation medium of callus was MS＋6-BA2 mg/L＋NAA0.2 mg/L. The optimal combination of hormones of buds differentiation was MS＋6-BA 0.4 mg/L. The effect of MS medium in radication culture was better than 1/2MS, the roots of plants were all efficiently induced in MS medium without reference to adding IBA, NAA or IBA＋NAA, the rate of radication was high. The effect of seedling cultured in the mixture of perlite and turf soil was better than perlite, garden soil and fiver sands. The mixture of perlite and turf soil has the excellences such as ventilate, water conservation, nourishment and so on. The survival rate was higher and substance was also hale.

关 键 词：华南鳞盖蕨 组织培养 假植技术 

分 类 号：S604.3[农业科学—园艺学] S644.1