羌活药材的色谱指纹图谱研究  

Studies on chromatographic fingerprints of Rhizoma et Radix Notopterygii

作　　者：古丽娜.沙比尔 崔亚君[1] 郭慧[1] 王炜[1] 果德安[1] 

机构地区：[1]北京大学药学院

出　　处：《中国中药杂志》2007年第1期30-33,共4页China Journal of Chinese Materia Medica

摘　　要：目的:建立羌活药材的色谱指纹图谱,为评价羌活药材的质量提供依据。方法:采用Phenom enex Lu-na C18(2)(4.6 mm×250 mm,5μm)色谱柱,乙腈(A)-0.3%醋酸水(B)为流动相梯度洗脱,流速1 mL.m in-1,检测波长330 nm,柱温30℃,洗脱时间70 m in。结果:得到分离度较好的羌活HPLC-UV色谱指纹图谱,标示了7个共有峰。并采用对照品比对和HPLC-MS技术对其部分色谱峰进行了定性鉴别。结论:色谱指纹图谱分析方法为全面控制羌活药材的质量提供了依据。Objective： To establish the chromatographic fingerprints for the identification and quality evaluation of Rhizoma et Radix Notopterygii. Method： Separation was performed on Phenomenex Luna C18 （2） （4.6 mm ×250 mm, 5 p.m） column, with mobile phase consisting of acetonitrile （A） and water containing 0. 3% acetic acid （B） with gradient elution at the flow rate of 1 mL ·mm . The UV detection wavelength was 330 nm, column temperature was 30 ℃, and the analysis time was 70 rain. Result： A satisfactory fingerprint was obtained by HPLC method, and qualitative analysis of some of the peaks were carried on by HPLC/MS approach. Conclusion： The established method is simple and rapid to identity and differentiate samples of Nhizoma et Radix Notopterygii collected from different sources and might be used as a quality control method for Rhizoma et Radix Notopterygii.

关 键 词：羌活 HPLC—UV—MS 色谱指纹图谱 

分 类 号：R284.1[医药卫生—中药学]