清开灵防治化学性肺水肿的有效成分追踪和作用机制研究  

A study of mechanism and effective ingredients of Qingkailing for PFIB inhalation induced pulmonary edema

作　　者：张天宏[1] 邵志华 林娟 徐进 丁日高[1] 阮金秀[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]浙江省衢州市衢化医院,浙江衢州324004 [3]山东省烟台市市直机关医院,山东烟台264000

出　　处：《中国中药杂志》2007年第1期53-57,共5页China Journal of Chinese Materia Medica

摘　　要：目的:揭示清开灵防治化学性肺水肿的有效成分及可能的作用机制。方法:雄性ICR小鼠随机分组,全氟异丁烯(PFIB)动态吸入染毒后1 h分别腹腔注射清开灵的4个部位和全方,观察染毒后24 h肺系数等指标变化。结果:①清开灵对抗PFIB所致肺水肿作用的有效部位是总胆酸组分。②清开灵方中非胆酸组分对总胆酸组分无促进协同作用。总胆酸组分中以猪胆酸的效果稍强,与牛胆酸配伍后,无相互协同作用。③PFIB吸入肺水肿发生机制与二甲苯致炎机制不同,表现为糖皮质激素可有效抑制二甲苯致炎作用,但无对抗PFIB肺水肿作用,说明在化学性肺水肿的治疗中,清开灵具有非激素样的独特的作用机制。④这种独特的作用机制可能与表面活性作用相关。结论:清开灵的作用机制可能与胆酸的表面活性作用有关。Objective： To unveil the effective ingredients and mechanisms of Qingkailing injection on the treatment of pulmonary edema induced by chemicals. Method： Mice were exposed to perfluoro sobutylene（PFIB） in a flow-pass chamber, the lung index after 24 h were determined. Result： ① The part of total cholic acid played a pivotal role; ② The parts of non-cholic acid had no cooperative effects to the part of cholic acid if co-administration. In the part of total cholic acid, the efficiency of hyodeoxylcholic acid was better than that of cholic acid; ③ Qingkailing injection would have special mechanism different from corticosteroid, which has no effect against PFIB but dimethylbenzene; ④ The special effects of cholic acid maybe result from its surfactant effects. Conclusion： The mechanism of Qingkailing injection may be related to the cholic acid due to its surfactant effects.

关 键 词：肺水肿 清开灵 胆酸 表面活性剂 

分 类 号：R285.5[医药卫生—中药学]