瓜叶菊耐热无性系Z_(1-1-1)的离体筛选与初步鉴定  

In Vitro Selection and Identification of Heat-Tolerant Clonal Line Z_(1-1-1)of Senecio cruentus

作　　者：于晓英[1] 卢向阳[2] 姚觉[1] 邱收[1] 李达[1] 陈永华[3] 

机构地区：[1]湖南农业大学园艺园林学院,长沙410126 [2]湖南农业大学生化与发酵工程实验室,长沙410128 [3]中南林业科技大学资源与环境学院,长沙410004

出　　处：《林业科学》2006年第12期33-38,I0002,共7页Scientia Silvae Sinicae

基　　金：湖南省教育厅资助项目(04C286)。

摘　　要：以瓜叶菊增殖期的愈伤组织和丛生芽为材料，用高温胁迫和羟脯氨酸（HYP）分别作为直接和间接选择压，进行瓜叶菊耐热无性系的离体筛选。结果表明：1）40℃16—20h胁迫处理可以作为瓜叶菊耐热无性系离体直接筛选的适宜选择压，30mmol·L^-1。HYP可以作为瓜叶菊耐热无性系离体间接筛选的适宜浓度；2）经过高温间隔胁迫多代筛选获得了与其母株相比耐热性有了很大提高的无性系Z1-1-1；3）Z1-1-1和其对照母株Z1的30d移栽苗在叶片厚度、气孔指数、栅栏组织厚度，海绵组织厚度方面存在显著差异，另外，成年植株Z1-1-1和Z1在花的育性上差异明显，Z1结实率85％以上，而Z1-1-1的结实率在人工授粉的情况下也仅1．0％；4）在40℃24h胁迫后，Z1-1-1的热害指数、细胞丙二醛（MDA）含量、电解质伤害性外漏率远远低于其对照母株，而POD、SOD活性明显高于其对照母株。存在显著性差异。In vitro selection of heat-tolerant clonal lines in Senecio cruentus was studied using tussock plantlets and callus as materials and high temperature and HYP as direct and indirect selection stress respectively. The results showed that ： 1） 40 ℃ 16- 20 h and 30 mmol· L^-1 HYP were proper direct and indirect selection stress pressure respectively. 2） One clonal line Z1-1-1 were obtained through high temperature stress selection, and the regenerated plants of Z1-1-1 were more tolerant to high temperature than their explants source genotype （ Z1 ） . 3） After transplanted for 30 d, the regenerated plants of Z1-1 - 1 were different significantly from their explants source genotype（Z1 ） in the thickness of leaves, the thickness ratio of palisade tissue to spongy tissue（TPT/TST） and the stomatal index. After artificial pollination, the fruit bearing percentage of adult plant of Z1-1-1 was only 1.0 % , and Z1 ＇ s was over 85 %. 4） After heat stress （40 ℃ 24 h）, the heat injury index, content of MDA and electrolyte leakage（ IL）of the regenerated plants of Z1-1-1 were lower than those of explants source genotype（ Z1 ）, but the POD, SOD activity was higher than that of Z1 .

关 键 词：瓜叶菊 耐热无性系 离体筛选 

分 类 号：S722.37[农业科学—林木遗传育种] Q943.1[农业科学—林学]