水曲柳体细胞胚与合子胚发生的细胞学研究  

Cytological Investigation of Somatic and Zygotic Embryogenesis of Fraxinus mandshurica

作　　者：孔冬梅[1] 沈海龙[2] 冯丹丹[2] 张莉杰[2] 

机构地区：[1]山西大学生命科学与技术学院,太原030006 [2]东北林业大学林学院,哈尔滨150040

出　　处：《林业科学》2006年第12期130-133,F0003,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金主任基金(30040041);面上项目(30170072)。

摘　　要：Maturing seeds were collected from fifty-year-old trees of Fraxinus mandshurica. The cotyledon and hypocotyl zygotic embryos were excised and cultured on 1/2MS media to induce somatic embryos. In cytological study of embryo development, by the way of paraffin, somatic and zygotic embryogenesis was observed and compared under light microscope. The results showed that somatic embryos from zygotic cotyledons originated directly from single epidermis cell. The somatic embryos that indirectly occurred on the embryogenic callus came from its singular surface cell or internal multi_cells. There was an obvious cytological difference between embryogenic and non-embryogenic callus. Somatic and zygotic embryogenesis underwent the similar course, passing through proembryo, heart, torpedo and cotyledonary stages. The important differences in morphogenetic process exited as following: volume of somatic embryos was smaller than its partner at the same development stage; somatic embryos had no obvious suspensors; somatic embryos showed morphologic variations.Maturing seeds were collected from fifty-year-old trees of Fraxinus mandshurica. The cotyledon and hypocotyl zygotic embryos were excised and cultured on 1/2MS media to induce somatic embryos. In cytological study of embryo development, by the way of paraffin, somatic and zygotic embryogenesis was observed and compared under light microscope. The results showed that somatic embryos from zygotic cotyledons originated directly from single epidermis cell. The somatic embryos that indirectly occurred on the embryogenic callus came from its singular surface cell or internal multi-cells. There was an obvious cytological difference between embryogenic and non-embryogenic callus. Somatic and zygotic embryogenesis underwent the similar course, passing through proembryo, heart, torpedo and cotyledonary stages. The important differences in morphogenetic process exited as following： volume of somatic embryos was smaller than its partner at the same development stage; somatic embryos had no obvious suspensors; somatic embryos showed morphologic variations.

关 键 词：水曲柳 体细胞胚发生 合子胚发生 细胞学观察 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]