广西吸毒者、肝病患者及献血员丙型肝炎病毒血清学基因型和基因型的分子生物学研究  

Molecular biological research of hepatitis C virus (HCV) serotypes and genotypes among intravenous drug abusers (IVDAs), patients with liver diseases (PLDs) and blood donors (BDs) in Guangxi Province, Southern China

作　　者：葛宪民[1] 王树声[1] 李丹亚[2] 沟上雅史 吴蓉蓉 黄果勇[1] 李茵[2] 王缨[2] 曹堃 潘海东[1] 王红[1] 周开姣[1] 章玲珠[1] 钱小燕[1] 哲户悦朗 大野智仪 铃木馨 大羽健一 

机构地区：[1]广西壮族自治区卫生防疫站,530021 [2]广西医科大学 [3]日本国名古屋市立大学医学院

出　　处：《中华实验和临床病毒学杂志》1996年第4期312-317,共6页Chinese Journal of Experimental and Clinical Virology

摘　　要：选择283名静脉注射(静注)吸毒者、103例肝病患者和355名献血员(其中有110名为丙型肝炎病毒抗体阳性)进行丙型肝炎病毒(HCV)抗体免疫学检测、HCV血清学基因型和基因型的分子生物学研究。结果表明:283名静注吸毒者、103例肝病患者和355名献血员的抗-HCV阳性检出率分别为97.2%(275/283)、11.7%(12/103)和4.6%(11/240)。这三组人群的HCV血清学基因型Ⅰ型者分别为221/270(81.9%)、4/12(33.3%)和60/91(65.9%);HCV基因型在119例HCV感染者的静注吸毒者的HCV基因型1a、1b、2a、2b、3a、3b、4a、5a、6a和6b分别检出率为28.6%(34/119)、38.7%(46/119)、10.9%(13/119)、14.3%(17/119)、26.9%(32/119)、40.3%(48/119)、0(0/119)、0(0/119)、8.4%(10/119)、26.7%(31/119);12例HCV肝病患者检出1a为50.0%(6/12)、1b为100.0%(12/12)和3a为8.3%(1/12);在41例HCV感染的献血员中1a为24.4%(10/41)、1b为80.5%(33/41)、2b为2.4%(1/41)和3a为7.3%(3/41),5例(12.2%)不能确定型别。Guangxi is well known as a high epidemic area of hepatitis B virus (HBV) and liver diseases, but there was no report about hepatitis C virus (HCV). To investigate the prevalence of HCV, HCV serotypes and genotypes among Ⅳ-DAs, PLDs and BDs in Guangxi, 283 IVDAs, 103 PLDs and 355 BDs were enrolled in this study, and were detected for anti-HCV, HCV serotypes, genotypes and were sequenced. Results: 275/283(97.2% ) IVDAs, 12/103(11.7% ) PLDs and 11/240(4.6%) BDs were seropositive for anti-HCV. 221 (81.9%), 4 (33.3%) and 60(65.9%) were HCV Serotype 1 among 270 IVDAs, 12 PLDs and 91 BDs with HCV infection, respectively. 34(28.6% ), 46(38.7 % ), 13(10, 9%), 17(14.3%), 32(26.9%), 48(40.3%), 10(8.4%)and 31(26.7%)wereHCVGenotype 1a, 1b, 2a, 2b, 3a, 3h, 6a, and 6b among 119 IVDAs, respectively. 6(50.0% ) and 12(100.0% ) and 1(8.3% ) were HCV Genotype 1a, 1h, and 3a among12 PLDs, respectively. 10(24.4%) 33(80.5%), 1(2.4%), 3(7.3%) and 5(12.2%) were HCV Ganotype 1a, 1b, 2b, 3a and unidentified among 41 BDs, respectively. We determined HCV genotypes of all samples by PCR and/or Sequencing mthods using specific primers of HCV genotypes.

关 键 词：吸毒者 献血员 丙型肝炎病毒 分子生物学 

分 类 号：R512.63[医药卫生—内科学]