艾滋病血清学诊断新方法的建立  

Establishment of a new methot for detection of antibody to human immunodeficiency virus (HIV)

作　　者：皮国华[1] 温丽君[1] 谷淑燕[1] 邓瑶[1] 张素香[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052

出　　处：《中华实验和临床病毒学杂志》1996年第4期368-370,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：以基因工程表达的人免疫缺陷病毒1型(HIV-1)gp41和HIV-2型gp36作为诊断抗原,将抗原机械喷制在硝基纤维素膜上,以辣根过氧化物酶结合的兔抗人IgG作为标记抗体,建立了检测人血清中HIV-1/2型抗体的免疫斑点法(IDA)。用IDA检测血清中的HIV抗体,特异性和敏感性均达到国、内外ELISA检测水平,并完全达到对HIV-1/2型抗体诊断试剂国家质控标准。An immunodot assay (IDA) was established for the detection of IgG antibody to HIV-1 (gp41) and HIV-2 (GP36) antigens. A strip with purified gp41 and gp36 antigens expressed from bacteria was used. The IDA assay consiste of two steps involving test serum and horseradish-peroxidase- conjugated rabbit anti- human IgG antibody. This IDA is a sensitive, specific, convenient and rapid test for serological screening of HIV antibody, especially in field studies.

关 键 词：人免疫缺陷病毒 免疫斑点法 诊断 

分 类 号：R512.910.4[医药卫生—内科学]