高灵敏度分光光度法测定乳酸氟哌酸的研究与应用  

STUDY ON HIGHLY SENSITIVE SPECTROPHOTOMETRIC DETERMINATION OF NORFLOXACINI LACTAS AND ITS APPLICATION

作　　者：李红兵 李生泉[1] 杨凤仙[1] 黄晋果[1] 

机构地区：[1]山西省生物制品厂,山西农业大学中心实验室,山西农业大学校医院

出　　处：《山西农业大学学报》1996年第2期194-196,共3页

摘　　要：本文较详细研究了乳酸氟哌酸—锆（Ⅳ）—四溴二氯萤光素三元络合物。在可见光谱区，创立了一种简便、快速、测定痕量乳酸氟哌酸的新方法。络合物的最大吸收峰在５５６ｎｍ处。表观摩尔吸光系数ε＝２．５８×１０４Ｌ·ｍｏｌ－１·ｃｍ－１。乳酸氟哌酸含量在０～７０μｇ／１０ｍｌ范围内遵守比尔定律。方法用于兽药中乳酸氟哌酸的测定，结果令人满意。A new,simple and rapid spectrophotometric method for determination of trace Norfloxacini Lactas (NFL) with tetrabromodichloro-fluorescein(TBDCF) in the presebce of zirconium has been developed.The maxinum absorption of the complex of NFL-Zr (IV)-TBDCF is at 556 nm and its apparent molar absotptivity is 2.58×104L.mol-1.cm-1. Beer's law is obeyed br TBDCF in the range 0-70μg/10ml. Thg proposed method has been applied to determination of TBDCF in veterinary medicines with satisfactory results.

关 键 词：分光光度法 乳酸氟哌酸 测定 

分 类 号：R978.1[医药卫生—药品] R927[医药卫生—药学]