慢性阻塞性肺疾病大鼠气道炎症细胞及黏蛋白检测  

Detection of airway inflammation and Mucin5AC in rats with chronic obstructive pulmonary disease

作　　者：何燕霞[1] 巨春荣[2] 宋改芳[1] 王俊萍[3] 李良臣[3] 

机构地区：[1]郑州大学第二附属医院检验科,郑州450014 [2]广州医学院第一附属医院广州呼吸疾病研究所,广州510120 [3]郑州大学基础医学院生物化学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2007年第1期123-126,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:制备大鼠慢性阻塞性肺疾病(COPD)模型,对支气管肺泡灌洗液(BALF)进行细胞学检查,并观察黏蛋白(Mucin5AC)的表达。方法:20只Wistar大鼠随机均分为健康对照组和COPD组。以气管内注射药物和吸烟方法制备COPD模型,6周后收集BALF进行细胞学检查,以免疫组化方法观察呼吸道杯状细胞肥大情况及Mu-cin5AC的表达。结果:①BALF检查:健康对照组中性粒细胞数为(17.58±3.72)×106L-1,COPD组为(251.10±42.76)×106L-1,2组差异有统计学意义。②气道炎症病理学评分:健康对照组为(18.56±2.46)分,COPD组为(69.30±4.99)分,2组差异有统计学意义。③肺泡腔直径:健康对照组为(47.22±12.95)μm,COPD组为(92.58±4.32)μm,2组差异有统计学意义。④杯状细胞化生指数:健康对照组为(5.12±3.07)个/mm2,COPD组为(51.20±9.23)个/mm2,2组差异有统计学意义;⑤Mucin5AC阳性染色面积:健康对照组为(3.12±2.60)%,COPD组为(34.50±6.52)%,差异有统计学意义。BALF中的中性粒细胞计数与气道炎症病理学评分呈正相关。结论:多种炎症细胞参与了COPD发生及发展。Aim： To detect the airway inflammation and Mucin5AC in rats with chronic obstructive pulmonary disease （COPD）, Methods：Twenty Wistar rats were divided randomly into 2 groups： normal group （group N, n = 10） and the model of COPD group （group C, n = 10）. The COPD model was established through passive smoking and intratracheal instillation of LPS. BALF was collected to count inflammatory cells after 6 weeks. Immunohistoehemistry was processed to observe the goblet cell and the expression of Mucin5AC in airway tissue of the rats, Results： BALF analysis presented that PMN count was higher in group C （（251. 10 ±42.76） × 10^6 L^-1） than that in group N （（17.58 ±3.72）× 10^6 L^-1） and there was significant difference ; airway pathological score was higher in group C （ 69.30 ±4.99 ） than that in group N （18.56± 2. 46） and there was significant difference; alveolar diameter was longer in group C （ （92.58 ±4.32）p,m） than that in group N （ （47.22 ± 12.95 ）μm） and there was significant difference. DAB staining presented that goblet cell hyperplasia index in group C （ （ 51.20 ± 9.23）/mm^2 ） was higher than that in group N （ （5.12 ± 3.07 ）/mm^2 ） and there was significant difference; Mucin5AC was overexpressed in group C （（34. 50 ± 6.52）% ） and almost absent in group N （（3. 12 ± 2.60）% ） and there was significant difference. The PMN count was correlated with the airway pathological score. Conclusion： The results suggest that many inflammatory cells involve in the development and progress of COPD.

关 键 词：慢性阻塞性肺疾病 大鼠 中性粒细胞 黏蛋白 杯状细胞 

分 类 号：R56[医药卫生—呼吸系统]