核受体NR4A1蛋白在大鼠卵泡发育中的定位及其表达规律  

The localization and expression pattern of nuclear receptor NR4A1 protein in rat ovary

作　　者：翟永宁[1] 刁飞扬[1] 刘嘉茵[1] 

机构地区：[1]南京医科大学第一附属医院临床生殖医学中心,江苏南京210029

出　　处：《医学研究生学报》2007年第1期32-35,I0011,共5页Journal of Medical Postgraduates

基　　金：江苏省135重点学科基金项目(批准号:苏卫科教[2001]31号)

摘　　要：目的:通过研究核受体NR4A1蛋白在正常雌性SD大鼠不同卵巢功能周期和不同发育阶段卵泡及黄体细胞中的表达,推测其在卵泡发育中的生物学作用。方法:利用阴道涂片的方法,按不同的卵巢功能周期将实验大鼠24只分为四组,采集不同时期卵巢标本,采用免疫组织化学方法观察卵巢中NR4A1蛋白的定位及其表达情况。结果:NR4A1蛋白主要表达于卵巢卵泡膜细胞和黄体细胞胞质中,且在成熟卵泡卵泡膜细胞的表达量显著高于生长卵泡(P<0.01);在新鲜黄体细胞中表达量最高,显著高于动情前期和动情期的生长卵泡和成熟卵泡以及陈旧黄体细胞中的表达水平(P<0.01)。结论:核受体NR4A1蛋白表达与卵泡的发育、成熟、闭锁、黄体萎缩有明显的相关性。To investigate the localization and expression pattern of nuclear receptor NR4A1 protein in follicle and corpus luteum cells at different estrous stages in cycling female SD rat. Methods ：The SD experimental rats were divided into four ovarian stages based on the vaginal lavages, from which the ovaries were obtained in every cycling stage. The localization and expression pattern of NR4A1 in rat ovaries were characterized by immunohistochemistry. Results：NR4A1 was mainly expressed in the cytosol of ovarian theca cells and lutein ceils. Expression level of NR4A1 in theca cell of mature follicle was significantly higher than that in the growing follicle （ P 〈 0.01 ）. NR4A1 expression levels were the highest in new corpus luteum, and significantly higher than that in the old corpus luteum（ P 〈 0.01 ）. Conclusion ：NR4A1 expression was significantly correlated with follicular development and corpus luteum＇ s atresia.

关 键 词：核受体NR4A1 卵巢 卵泡膜细胞 黄体细胞 

分 类 号：R711.6[医药卫生—妇产科学]