人ACAT1基因P1启动子的克隆及意义  

Cloning of P1 promoter of human acyl coenzyme A: cholesterol acyltransferase1 gene

作　　者：葛晶[1] 成蓓[1] 文晖[1] 何平[1] 柯丽[1] 余其振[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《山东医药》2007年第2期4-6,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30471921)。

摘　　要：目的克隆人酰基辅酶A:胆固醇酰基转移酶1(ACAT 1)基因P 1启动子。方法应用PCR方法从人单核细胞系THP-1扩增分离出ACAT 1基因P 1启动子全长片段,将PCR产物克隆入T载体,并对所获得的序列进行生物信息学分析。结果经琼脂糖凝胶电泳及直接测序鉴定,克隆的ACAT 1基因P 1启动子片段碱基序列与G enB ank数据库一致,未发现突变。结论人ACAT 1基因P 1启动子克隆成功,此为动脉粥样硬化(A S)过程中ACAT 1基因转录调控机制的研究奠定了基础。[Objective] To clone P1 promoter of the human acyl eoenzyme A： cholesterol acyltransferasel1 （ACAT1） gene. [Methods] The complete nucleotide segment of P1 promoter was obtained from human monocytic leukemia cell line （THP-1） by PCR method. The PCR product was inserted into pMD19-T simple vector, and the sequence was analyzed by biological information method. [Results] The DNA sequence of P1 promoter cloned was accordant with GenBank data, and no mutation was found. [Conclusions] P1 promoter of the ACAT1 gene can be cloned successfully. These results may make an important basis for studying transcriptional regulation mechanisms of ACAT1 during atherosclerosis.

关 键 词：酰基辅酶A:胆固醇酰基转移酶1 基因 启动子区(遗传学) 克隆 

分 类 号：R392.2[医药卫生—免疫学]