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作 者:秦志华[1] 李健[1] 王芳[2] 刘淇[1] 王群[1]
机构地区:[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国海洋大学生命科学与技术学部,山东青岛266003
出 处:《水产科学》2007年第1期17-21,共5页Fisheries Science
基 金:国家"863"计划项目(2004AA603320);国家科技攻关计划(2004BA526B02);科技部社会公益研究专项(2004DIB4J165)
摘 要:用添加了不同含量Vc-2-多聚磷酸酯(600 mg/kg,3000 mg/kg、6000 mg/kg,对照)的饲料分别投喂中国对虾幼体(Z1-P5),测定其对幼体生长、变态、非特异性免疫以及体内Vc积累量的影响。结果显示:6000 mg/kg处理组显著促进了幼体的生长和变态(P<0.05);超氧化物岐化酶和抗菌活力较对照组和600 mg/kg处理组均达到了显著水平(P<0.05)。第20 d用副溶血弧菌攻毒,3000、6000mg/kg处理组均显著提高了幼体存活率;高效液相色谱法检测幼体体内的Vc含量发现,其体内Vc含量随着饲料中Vc-2-多聚磷酸酯含量的增加而增加。以生长、变态、超氧化物岐化酶、抗菌活力为评价指标,建议中国对虾幼体饲料中Vc-2-多聚磷酸酯的添加量为6000 mg/kg。The larvae of Chinese shrimp (Penaeus chinensis) of LAPP L-ascorbyl-2-polyphosphate. Growth, metamorphosis, were provided with diets containing different levels non-specific immunity, body vitamin C concentrations and resistance to Vibrio parahaemolyticus were investigated in the study. Results showed that the LAPP at 6000 mg/kg significantly enhanced growth and metamorphosis; The antibacterial activity and superoxide dismutase were significantly enhanced when the shrimp fed diet Containing 6000 mg/kg of LAPP; larvae fed 3000 mg/kg and 6000 mg/kg of LAPP hed significantly higher survival rate than those treated with 600 mg/kg and the control diet after challenged with V. parahaemolyticus. HPLC techniques tested quantification of vitamin C in shrimp body, and the results showed that the body vitamin C content increased with the increasing dietary level of LAPP. It appears that high levels of LAPP could promote growth, metamorphosis, and non-special immune competence.
关 键 词:Vc-2-多聚磷酸酯 中国对虾 幼体
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