泥蚶血细胞耐饥饿及抗菌力特性的研究  

Phagocytosis and Microstructure of Haemocytes of Tegillarca granosa Linnaeus

作　　者：潘沙芳[1] 李太武[1] 苏秀榕[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《水产科学》2007年第1期22-25,共4页Fisheries Science

基　　金：国家自然科学基金资助项目(30371095)

摘　　要：显微镜下观察泥蚶血细胞抗菌能力以及在不同饥饿条件下细胞形态结构的变化。结果表明:经饥饿处理后,泥蚶血细胞随着时间的迁移,出现细胞形态不规则、胞质色泽变浅、胞质颗粒物减少、胞膜边缘光滑度减小等现象。血细胞对大肠杆菌、奥斯陆莫拉氏菌、脲放线杆菌均具较高的噬菌率,噬菌实验过程中血细胞形态结构以及细胞组分不断产生变化。细胞抗菌活性检测结果表明:血细胞对三者都具有一定的抗菌活性,活性依次为:大肠杆菌>奥斯陆莫拉氏菌>脲放线杆菌。Cell microstructure at different starvation states and phagocytosis of haemocytes of TegiUarca granosa Linnaeus were studied by microscopic examination. According to microscopic observation with the Giemsa stain, the configuration of haemocytes became non-regulation, and granules in cytoplasm and smooth degree of cell membrane in rim were reduced gradually after starvation treatment. The haemocytes of Tegillarca granosa had strong phagocytic activities on Escherichia coli, Moraxella osloensis and Actinobacillus ureae. The configuration and components of haemocytes changed in different became smaller, the small granules in cytoplasm stages. In the first stage, as bacteria being digested, haemocytes reduced gradually, and granules of part haemocytes had exosmosis phenomenon. In phagocytic anaphase, big granules in cytoplasm assembled armund nucleolus. The antibacterial activities in the haemocytes of Tegillarca granosa were detected. The results showed that haemocytes had strong antibacterial activities on E. coli, Moraxella osloensis and Actinobacillus ureae. Their average antibacterial activities were E. col （ U ：0.2881 ） 〉 Moraxella osloensis （ U ：0. 2157 ） 〉 ActinobaciUus ureae （ U ：0.1143 ）.

关 键 词：泥蚶 血细胞 噬菌作用 

分 类 号：S968.314[农业科学—水产养殖]