肝素化胶原/丝素共混膜的制备及其抗凝血性能  

Preparation and anticoagulant activity of heparinized collagen/silk fibroin blend film

作　　者：程忠玲[1] 

机构地区：[1]承德石油高等专科学校化工系,河北省承德市067000

出　　处：《中国组织工程研究与临床康复》2007年第1期11-13,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：河北省科学技术研究与发展指导项目(05215512)~~

摘　　要：目的:制备一种新型肝素化胶原/丝素共混膜,观察其抗凝血性能。方法:实验于2003-12/2005-04在北京理工大学材料中心实验室完成。①将50mg胶原溶于0.2g/L醋酸溶液5mL中,然后滴加10g/L肝素溶液0.5mL,均匀搅拌10min,得到肝素化胶原。采用EQINOX55型红外光谱仪(美国NICOLET公司)进行结构表征。②将肝素化胶原与丝素溶液共混(质量比为1∶4),加入戊二醛溶液作交联剂,注入聚乙烯模具内干燥72h得肝素化胶原/丝素膜。测定肝素的标准曲线以及不同戊二醛用量对肝素的结合率和膜的力学性能的影响。③采用体外凝血时间试验评价材料的抗凝血性,包括活化部分凝血活酶时间、凝血酶时间、凝血活酶时间3种凝血时间测定,在COAG-A-MATE-XM型半自动四通道凝血仪(美国OrganonTekinika公司)中进行。结果:①肝素化胶原红外光谱在850cm-1处出现新峰。②当加入5g/L戊二醛溶液0.1mL时,所得共混膜中肝素的结合率为56%,共混膜的断裂强度为45MPa。③共混膜的活化部分凝血活酶时间、凝血酶时间、凝血活酶时间分别超过150,200,50s。结论:肝素化胶原与丝素共混膜中的肝素仍保持良好的抗凝血性。从而得到一种性能优良的新型生物材料。AIM： To prepare a new type heparinized collagen/silk fibroin blend film and investigate its anticoagulant activity. METHODS： The experiment was conducted at the Research Center of Material Science, Beijing Institute of Technology from December 2003 to April 2005. ①50 mg collagen was dissolved in 5 mL acetic acid solution （0.2 g/L）, and 0.5 mL heparin solution （10 g/L） was added and stirred for 10 minutes to prepare the heparinized collagen. The structural characteristics were examined through a infrared spectrometer （EQINOX55）. ②Glutaraldehyde as a coupling agent was added into the aqueous solution of heparinized collagen and silk flbroin （mass ratio of 1：4）, which was cast into a polystyrene plate. The blend film was formed after the plate dried for 72 hours. The standard curve, and the effect of glutaraldehyde at different doses on the combination rate of heparin and mechanical performance of film were measured. ③The blood anticoagulant activity of the blend film was assessed by in vitro coagulation time test including activated partial thromboplastin time （APTT）, thrombin time （TT） and activated thromboplastin time （PT） measurement, which were performed by a photo-optical clot detection instrument COAG-A-MATE-XM （Organon Tekinika Company, U.S.）. RESULTS ①Hepadnized collagen showed absorption bends at 850 cm^-1. ②The combination rate of the blend film approached to 56% and the rupture strength was 45 MPa as 0.1mL glutaraldehyde （5 g/L） was added. ③APTT, TT, and PT of the blend film was over 150, 200 and 50 seconds, respectively. CONCLUSION： The heparin in beparinized collagen/silk fibroin blend film still possesses good anticoagulant activity; it may be a new biomatedal with excellent performance.

关 键 词：胶原 丝素 肝素化 抗凝血性 

分 类 号：R318.08[医药卫生—生物医学工程]