聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨复合人工骨的体外降解  

Degradation of bone repairing composite of calcium polyphosphate fiber,calcium phosphate cement and micromorselized bone in vitro

作　　者：周磊[1] 闫景龙[1] 胡春杰[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨四科,黑龙江省哈尔滨市150081

出　　处：《中国组织工程研究与临床康复》2007年第1期33-36,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30371441)~~

摘　　要：目的:观察聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨复合材料的体外降解特性。方法:实验于2005-02/10在哈尔滨医科大学附属第一医院动物实验中心完成。①取兔髂骨制成直径300～500μm的微小颗粒骨。②分别按质量比聚磷酸钙纤维:磷酸钙骨水泥:微小颗粒骨=1∶4∶4(聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨组)和磷酸钙骨水泥:微小颗粒骨=1∶1(磷酸钙骨水泥/微小颗粒骨组)制成两组生物复合材料。②通过扫描电镜观察复合材料的孔径并计算孔隙率。③进行体外降解实验,将两组复合材料置于pH值7.4的磷酸盐缓冲液中,观察复合材料在降解液中失重率的变化,降解液pH值的变化及在不同降解时间复合材料生物力学的变化。结果:①两种复合材料孔隙率及溶液pH值、降解质量变化:聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨复合材料孔隙率为72.1%,孔径为100～400μm,降解过程中pH值稳定,4周内质量变化较小,4周后下降较快,12周时为初始质量的50%;磷酸钙骨水泥/微小颗粒骨组复合材料孔隙率为58.2%,孔径50～300μm,降解过程中pH值有轻度变化,6周内质量变化较小,6周后下降较快,12周时为初始质量的70%。②两种复合材料在降解过程中生物力学性能的变化:聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨复合材料初始抗压强度9.28MPa,磷酸钙骨水泥/微小颗粒骨复合材料初始抗压强度为6.21MPa,两者差异有显著性(P<0.05);聚磷酸钙纤维/磷酸钙骨水泥/颗粒骨强度在0～4周下降较慢,4周后下降较快,12周时为0.18MPa,磷酸钙骨水泥/颗粒骨强度均匀下降,12周时为0.24MPa。结论:聚磷酸钙纤维/磷酸钙骨水泥/微小颗粒骨具有良好孔隙率、孔径和降解性能,可能成为一种新型治疗骨缺损材料。AIM： To explore the degradation characteristics of composite of calcium polyphosphate fiber （CPPF）, calcium phosphate cement （CPC） and micromorselized bone in vitro. METHODS： The experiment was conducted in the Central Laboratory of Animal Experiment of the First Hospital Affiliated to Harbin Medical University from February to October 2005. ① The rabbit iliac bone was made into micromorselized bone with the diameter of 300-500 um. ② Two groups of biochemical composite were made respectively according to the mass-ratios of CPPF/CPC/micromorselized bone at 1：4：4 （CPPF group/CPC group/micromorselized bone group） and that of CPC/micromorselized bone at 1：1 （CPC group/micromorselized bone group）. ③ The porosity was calculated by studying the pore diameter of composite under scanning electron microscope. In vitro experiment of degradation, two groups of composite were put into the phosphate buffered solution （PBS） （pH 7.4） respectively to study the changes in weight-loss ratio of composite in degradation liquid as well as the changes in pH value of degradation liquid and the biomechanical changes in composite at different time-points. RESULTS ：① Changes in porosity, pH value and mass of degradation of both kinds of composites： The porosity of composite of CPPF/CPC/micromorselized bone was 72.1% with the pore diameter of 100-400, and the pH value was constant while degradation. The mass changed little within 4 weeks and decreased rapidly after the 4^th week, which was 50% of the initial mass in the 12^th week. The porosity of compound materials of CPC/micromorselized bone was 58.2% and was 50-300 um in the gap diameter with slight changes in pH value, and the mass changed little within 6 weeks, which decreased rapidly after 6 week, and was 70% of the initial mass in the 12= week. ② Biomechanical changes in composite of two groups in the degradation： the primary biomechanical streng th was 9.28 MPa in CPPF/CPC/micromorselized bone and was 6.21 MPa in CPC/micromorselized

关 键 词：磷酸钙类 骨代用品 生物降解 

分 类 号：R316.06[医药卫生—基础医学]