珊瑚羟基磷灰石/骨形态发生蛋白复合骨组织工程细胞支架的生物相容性(英文)  

Biocompatibility of coralline hydroxyapatite/bone morphogenic protein composite for bone tissue engineering

作　　者：尹飚[1] 张余[1] 张宏斌[1] 尹庆水[1] 

机构地区：[1]解放军广州军区广州总医院骨科,广东省广州市510010

出　　处：《中国组织工程研究与临床康复》2007年第1期191-193,F0003,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省医学科学技术研究基金(B2004100)~~

摘　　要：背景:理想的骨细胞支架材料除具有良好的生物相容性外,还能促进细胞的增殖和分化,诱导骨组织再生,各种细胞支架材料单独使用时的成骨能力是有限的。目的:观察复合有骨形态发生蛋白的珊瑚羟基磷灰石与成骨细胞的生物相容性。设计:对比观察。材料:珊瑚羟基磷灰石/骨形态发生蛋白、珊瑚羟基磷灰石,将其分别切割成4mm×4mm×3mm和10mm×10mm×3mm大小块状物,高压灭菌后,完全培养基浸泡4d后备用。方法:实验于2005-03/08在广州军区广州总医院中心实验室完成。①取新西兰大白兔骨髓成骨细胞培养。②将成骨细胞分别和珊瑚羟基磷灰石/骨形态发生蛋白(复合珊瑚羟基磷灰石组)、珊瑚羟基磷灰石(单纯珊瑚羟基磷灰石组)混合培养,并设空白对照。③混合培养后2,4,6,8d分别检测细胞增殖指数和碱性磷酸酶活性。主要观察指标:①成骨细胞相差显微镜观察结果。②复合珊瑚羟基磷灰石材料扫描电镜观察结果。③各组细胞碱性磷酸酶活性测定。④各组细胞增殖指数测定。结果:①成骨细胞相差显微镜观察结果:细胞呈梭形、扁圆形及多角形外观,有数量不等、长短及粗细不一的突起,细胞通过突起相互间接触。②复合珊瑚羟基磷灰石材料扫描电镜观察结果:材料表面呈颗粒状,具有微孔。成骨细胞在材料表面贴壁生长,初为单层,随着细胞的进一步分化、增殖,细胞开始叠层生长,细胞周围出现高密度颗粒,排列方式绝大多数呈一定取向,随着钙盐结晶的增多,最终形成骨结节。细胞被包埋在基质中。③各组细胞碱性磷酸酶活性测定显示:混合培养后2,4,6,8d复合珊瑚羟基磷灰石组的碱性磷酸酶活性(A值)明显高于单纯珊瑚羟基磷灰石组和对照组(4d:101.6±7.7,93.7±6.8,94.4±5.0;8d:120.1±8.2,108.3±8.8,110.4±7.5;P<0.01或0.05)。单纯珊瑚羟基磷灰石组与对照组比较差异不明显。④各组细胞�BACKGROUND： Besides good biocompatibility, ideal scaffold for bone tissue engineering should possess the characters of promoting cell proliferation and differentiation, inducing the regeneration of bone tissue. However, the osteogenetic ability is limited when the materials of scaffold is used alone. OBJECTIVE： To observe the biocompatibility of coralline hydroxyapatite （CHA）/bone morphogenic protein （BMP） composite and osteoblasts. DESTGN ： A comparative observation SETTING： Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Area Command Of Chinese PLA. MATERTALS： Composite of CHA/BMP （CCHA） and CHA were cut into blocks of 4 mm ×4 mm ×3 mm and 10 mm × 10 mm × 3 mm respectively, immersed in complete medium for 4 days after autoclaving. METHODS： The experiments were carded out in the Central Laboratory of the Guangzhou General Hospital of Military Area Command of Chinese PLA from March to August in 2005. ① The bone marrow of New Zealand rabbits were extracted to culture osteoblasts. ② The osteoblasts were co-cultured with CCHA （CCHA group） and CHA （CHA group） respectively, and the blank control group was untouched. ③ The proliferative index of osteoblasts and activity of alkaline phosphatase were measured at 2, 4, 6 and 8 days alter co-culture. NATN OUTCOME MEASURES ： ① Results of osteoblasts observed under phase contrast microscope; ② Results of CCHA material observed under scanning electron microscope; ③ Activity of alkaline phosphatase; ④ Proliferative index of os- teoblasts. RESULTS： ①Osteoblasts observed under phase contrast microscope： The cell appearance was fusiform, oblate and multi-angles with processes of various number and size, and the cells connected with each other by the processes. ② CCHA observed under scanning electron microscope： The CCHA surface was granule-like with micropores. The osteoblasts attached to the surface of the materials and grew in monolayer at first, then overlapped as the cells further diffe

关 键 词：羟基磷灰石类 成骨细胞 生物相容性材料 组织工程 

分 类 号：R318[医药卫生—生物医学工程]