阿德福韦酯治疗慢性乙型肝炎患者前后蛋白质组学的比较分析  

The serum proteomic analysis of the adefovir dipivoxil response in the chronic hepatitis B patients

作　　者：郭芳[1] 王江华[1] 饶慧瑛[1] 王松霞[1] 管文莉[1] 孙焱[1] 王豪[1] 魏来[1] 

机构地区：[1]北京大学人民医院,北京大学肝病研究所100044

出　　处：《中华检验医学杂志》2007年第1期48-52,共5页Chinese Journal of Laboratory Medicine

基　　金：国家重点基础研究发展规划(2005CB522902);北京市重大科技计划项目(H030230280410)

摘　　要：目的比较阿德福韦酯(adefovir dipivoxil,ADV)治疗前后有病毒学应答患者和无病毒学应答患者血清蛋白质组学变化差异,探讨预测 ADV 治疗效果的特异性的标志。方法利用双向凝胶电泳-质谱技术获得慢性乙型肝炎患者血清蛋白表达图谱,并寻找 ADV 治疗后差异表达的蛋白质。结果经与治疗前比较,有病毒学应答组触珠蛋白、触珠蛋白2α、α-1-抗胰蛋白酶前体在治疗后上调;因子 B、转甲状腺素 B 链、谷胱甘肽过氧化酶、α-2-巯基蛋白、视黄醇结合蛋白、视黄醇结合蛋白前体、载脂蛋白、载脂蛋白 A-I 前体在治疗后下调。无病毒学应答组转甲状腺素在治疗后上调;富含亮氨酸α-2-糖蛋白、触珠蛋白、α-2-肌动蛋白、载脂蛋白 A-I 前体在治疗后下调。两组相比:治疗后载脂蛋白 A-I 前体呈相同下调趋势;触珠蛋白、转甲状腺素表达呈相反变化趋势。结论双向电泳-质谱技术可发现 ADV 治疗过程后有病毒学应答组触珠蛋白、转甲状腺素表达与无病毒学应答组比较呈相反变化趋势,从而为寻找 ADV 治疗有效的预测指标奠定基础。Objective To compare the difference of the protein about the patient of hepatitis B who received adefovir dipivoxil（ADV） therapy, and seek the useful biomarker of effective therapy. Methods We used the two-dimensional gel electrophoresis technology to examine HBV infected serum samples aiming at searching protein＇s alteration after ADV therapy. Results After 1 year＇s treatment, haptoglohin, haptoglobin 2-alpha raised and alpha-l-antitrypsin precursor, Factor B, Chain B, transthyretin, glutathione peroxidnse, alpha-2-HS-glycoprotein, retinal binding protein, retinol-binding protein precursor, apolipoprotein, apolipoprotein A-I precursor fell in viral response patients. Transthyretin raised and leueinerich alpha-2-glyeoprotein, haptoglobin, alpha-2-actin, apolipoprotein A-I precursor fell in none viral response patients. To compare two groups： apolipoprotein A-I have the same change and haptoglohin, transthyretin have the opposite change Conclusion Proteemics study can find the alteration of protein during the ADV treatment, and is helpful to searching the predictable biomarker to ADV.

关 键 词：肝炎 乙型 慢性 蛋白质组学 电泳 凝胶 双向 

分 类 号：R512.6[医药卫生—内科学]