主动外排系统在耐甲氧西林金黄色葡萄球菌多重耐药中的作用  

The action of active effiux system on multi-drug resistance in methicillin resistant Staphylococcus aureus

作　　者：莫晓能[1] 李建国[1] 唐英春[2] 张永[2] 朱家馨[2] 谈淑卿[2] 陆坚[2] 

机构地区：[1]中山大学附属第二医院呼吸内科,广州510120 [2]中山大学附属第三医院

出　　处：《中华结核和呼吸杂志》2007年第1期40-43,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：广东省卫生厅科研基金(A2004552)

摘　　要：目的分析耐甲氧西林金黄色葡萄球菌(MRSA)主动外排基因(qacB、qacJ、smr),探讨其在 MRSA 多重耐药中的作用。方法采用计算机引物设计软件(Primer Premier 5.0)设计外排泵基因(qacB、qacJ、smr)引物,用 PCR 和电泳分析方法检测124株 MRSA 主动外排基因;用利血平进行MRSA 协同抑制试验,观察其对利福平和左旋氧氟沙星药敏性的变化。结果 124株 MRSA 中检出外排泵 qacB 基因86株,检出率为69.4%;qacJ 基因45株,检出率为36.3%;smr 基因32株,检出率为25.8%。利血平抑制实验结果显示 MRSA 对左旋氧氟沙星和利福平的最低抑菌浓度值下降2～32倍,表明其耐药性降低。结论 MRSA 存在 qacB、qacJ、smr 等多种主动外排系统,在其多重耐药中起重要作用。Objective To study the active efflux gene qacB, qacJ and star in methicillin resistant Staphylococcus aureus （MRSA） and to investigate their effect on the multi-drug resistance （MDR） of MRSA. Methods The three pairs of ideal primers of active efflux gene qacB, qacJ and smr were designed by computer with Primer Premier 5. 0 software. A total of 124 clinical isolates of MRSA were amplified respectively by polymerase chain reaction （PCR） with above mentioned primers. The PCR products were separated by electrophoresis on an 1.5% agarose gel with 0. 5 μg/nd ethidium bronide. Reserpine inhibition test was used to observe the changes of the susceptibility to antibiotics of MRSA which had qacB , qacJ and smr genes separately. Results Of the 124 strains of MRSA, 86 strains had qacB , 45 strains had qacJ and 32 strains had smr gene. Reserpine inhibition test showed that the minimal inhibitory concentration （MIC） decreased 2 to 32 times for MRSA to levofloxacin and rifampin. Conclusion MRSA have qacB, qacJ and smr active efflux systems, which play a very important role in multiple antibiotic resistance.

关 键 词：葡萄球菌 金黄色 主动外排基因 多重耐药 

分 类 号：R515[医药卫生—内科学]