细胞周期蛋白B1及Cdc2在正常子宫内膜及子宫内膜异位症患者在位和异位内膜组织中的表达  

Expression of cyclin B1 and cyclin-dependent kanasel in ectopic and eutopic endometrium tissues of patients with endometriosis

作　　者：唐莉[1] 何荣环[1] 周彩云[2] 潘虹[1] 黄荷凤[1] 

机构地区：[1]浙江大学医学院附属妇产科医院生殖内分泌科,杭州310006 [2]浙江大学医学院附属妇产科医院生殖病理科,杭州310006

出　　处：《中华医学杂志》2007年第3期179-183,共5页National Medical Journal of China

摘　　要：目的探讨细胞周期蛋白(CyclinB1)及 Cyclin-Dependent kinasel(Cdc2)在子宫内膜异位症发生发展中的作用及其与卵巢激素之间的相关关系。方法采用 RT-PCR,Western 印迹以及免疫组织化学的方法检测了子宫内膜异位症患者(异位症组)的异位内膜(29例)和在位子宫内膜(20例)以及正常生育期的其他良性疾病妇女的子宫内膜(对照组,30例)中 CychnB1及 Cdc2的表达。结果 CyclinB1在异位症组异位内膜上的表达量明显高于在位内膜及对照组(P<0.05)。异位症组在位内膜上 CyclinB1的表达量与对照组相比没有显著性差异(P>0.05)。Cdc2在各组患者中的表达差异无统计学意义(P>0.05)。结论 CyclinB1高表达可能与子宫内膜异位症异位内膜的增生相关。Objective To investigate the possible roles of cyclin B1 and cyclin-dependent kanasel cyclin-dependent kanasel play in the pathogenesis and development of endometriosis and their association with the ovarian hormones. Methods Twenty-nine specimens of ectopic endometrium tissues and 20 specimens of eutopic endometrium tissues were obtained from 29 patients with endometriosis, aged 24 - 46. Thirty specimens of endometrium form 30 women without endometriosis were used as controls. The intracellular location of cyclin B1 and Cdc2 was detected by microscopy. Western blotting, RT-PCR, and immunohistochemistry were employed to examine the mRNA expression and protein expression of cyclin B1 and Cdc2. Serum estrogen and progestogen were detected. Results The expression level of cyclin B1 in the ectopic endome.trium from the women with endometriosis was significantly higher than that in the eutopic endometrium tissues from the women with and without endometriosis （ both P 〈 0. 05 ）. No significant difference was found between the expression level of cyclin B1 in the eutopic endometrium tissues from the women with and without endometriosis （ both P 〉 0. 05 ）. The Cdc2 expression levels were not significantly different among th3 3 groups and the proliferative and secretary stage of endometrium （ all P 〉 0. 05 ）. Cyclin B1 expression level was positively correlated with the serum estrogen level, and negatively correlated with the serum progestogen level, and Cdc2 expression was not correlated with the serum sex hormone levels. Conclusion The expression of cyclin B1 in the ectopic endometrium is higher than normal. Cyclin B1 may be involved in the proliferation of endometrium in endometriosis.

关 键 词：子宫内膜异位症 细胞周期蛋白B1 异位组织 子宫内膜 

分 类 号：R711.71[医药卫生—妇产科学]