解毒化淤Ⅱ方中药配方颗粒剂对肝衰竭大鼠肝线粒体脂质过氧化的影响  

Influence of the Detoxification and Dissipation Blood Stasis Formula Ⅱ on Hepatic Mitochondria Lipid Peroxidation in Rats with Hepatic Failure

作　　者：毛德文[1] 邱华[1] 余晶[1] 马银瑶[1] 

机构地区：[1]广西中医学院第一附属医院中医肝病治疗中心,南宁市530023

出　　处：《中国药房》2007年第3期166-168,共3页China Pharmacy

基　　金：国家自然科学基金项目(30360125);广西自然科学基金项目(桂科自0542103)

摘　　要：目的研究解毒化淤Ⅱ方的中药配方颗粒对暴发性肝衰竭大鼠肝线粒体内脂质过氧化作用的影响。方法采用皮下注射硫代乙酰胺(TAA)法复制暴发性肝衰竭大鼠模型。取Wistar大鼠84只,随机分为空白组、模型组、解毒化淤Ⅱ方中药配方颗粒剂(低、中、高剂量组)、安宫牛黄丸组、乳果糖组,造模前3d开始灌胃给药,2次.d-1,间隔12h,共给药11次;造模完成12h后,测定各组大鼠肝线粒体内丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、一氧化氮(NO)及肝脏的坏死面积。结果解毒化淤Ⅱ方中药配方颗粒剂能显著减少肝脏坏死面积,抑制肝线粒体内脂质过氧化终产物MDA的生成,恢复SOD、CAT的活力,提高GSH、NO的含量,并呈量效关系,与模型组比较差异有统计学意义(P<0.05或P<0.01);肝脏的坏死面积与肝线粒体内MDA呈正相关关系,与SOD、CAT、GSH、NO呈负相关关系。结论解毒化淤Ⅱ方中药配方颗粒剂对暴发性肝衰竭肝细胞具有较强的保护作用,其作用机制可能是通过拮抗脂质过氧化来实现。OBJECTIVE： To investigate the influence of TCM Granules of the detoxification and dissipation blood stasis formula Ⅱ on fulminant hepatic mitochondrial lipid peroxidation in rats with hepatic failure. METHODS： The fulminant hepatic failure rat models were established by subcutaneous injection of thioacetamlde （TAA） . 48 Wistar rats were randomly divided into 7 groups： blank control group, model group, low dose group, medium dose group, high dose group, the lactulose treatment group, and the ＂ Bezoar pill for resurrection＂ treatment group. Intragastric administration was executed 3 d before model making, twice per day with the interval of 12 hours. They were administered for lltimes. 12 hours after model establishment. MDA, SOD, CAT, GSH, NO and liver necrosis area in hepatic mitochondria were determined. RESULTS： The detoxification and dissipation blood stasis formula Ⅱ could notably reduce liver necrosis area and restrain produce of hepatic mitochondrial lipid peroxidatide MDA, retrieve the activity of SOD, CAT and increase the content of GSH, NO. Moreover it shows doseeffect relation. Compared with model group, there is statistical significance（P〈0.05 or P〈 0.01） .The liver necrosis area was positively correlated with hepatic mitochondria MDA, but negatively correlated with SOD,CAT,GSH,NO . CONCLUSION： The detoxification and dissipation blood stasis formula Ⅱ protects hepatocytes in rats with fulminant hepatic failure. This may be due to the resistance to hepatic mitochondrial lipid peroxidation.

关 键 词：解毒化淤Ⅱ方 暴发性肝衰竭 脂质过氧化 肝线粒体 中药配方颗粒剂 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]