不同制备工艺板蓝根多糖对小鼠脾淋巴细胞增殖作用的影响  

Influence of Radix Isatidis Polysaccharides Produced by Different Preparation technologies on Proliferation Function of Mouse Splenic Lymphocyte

作　　者：马莉[1,2] 冯少华[1,3] 唐健元[4] 赵艳玲[1] 肖小河[1] 

机构地区：[1]解放军302医院中医药研究所 [2]首都医科大学中医药学院,北京市100069 [3]天津大学药物科学与技术学院,天津市300072 [4]国家食品药品监督管理局药品审评中心

出　　处：《中国药房》2007年第3期169-171,共3页China Pharmacy

基　　金：国家自然科学基金项目(39970911)

摘　　要：目的研究板蓝根在不同制备工艺条件下得到的多糖对小鼠脾淋巴细胞增殖的影响。方法用不同浓度乙醇将板蓝根水提液进行分级沉淀,并结合二乙基氨基乙基纤维素离子交换层析柱,凝胶色谱柱分离纯化得到IP-Ⅰ、IP-Ⅱ、IP-Ⅲ…IP-Ⅷ8个粗多糖部分及IP-Ⅸ、IP-Ⅹ、IP-Ⅵ、IP-Ⅻ4个均一多糖部分。采用四甲基偶氮唑蓝法检测板蓝根多糖对小鼠脾细胞增殖反应的影响。结果板蓝根多糖各部分均具有促进淋巴细胞增殖作用,且存在一定的量效关系。结论板蓝根多糖对淋巴细胞的促增殖作用的最适剂量为IP-Ⅸ(0.125mg.mL-1)和IP-Ⅻ(0.125mg.mL-1)。OBJECTIVE： To study the influence of Radix Isatidis polysaccharides produced by different preparation technologies on proliferation function of mouse splenic lymphocyte. METHODS： Water extracts of Radix Isatidis had fractional precipitation by ethanol of different concentrations. Eight crude polysaccharide parts of IP-Ⅰ、IP-Ⅱ、IPⅢ…IP-Ⅷ and four homogeneous polysaccharide parts of IP-Ⅸ、IP-Ⅹ、IP-Ⅵ、IP-Ⅻ were obtained after fractionated by DEAE cellulose ion exchange chromatography and gel chromatography, Influence on proliferation function of mouse splenic lymphocyte was determined by MTT method. RESULTS.. It showed that all polysaccharide parts of Radix Isatidls could promote the prolif- eration function of mouse splenic lymphocyte and dose -effect relationship existed. CONCLUSION： The optimum dose of polysaccharide parts was IP- Ⅸ（0.125mg· mL^ -1） and IP-Ⅻ （0.125mg · mL^ -1）, which can promote the proliferation of lymphocyte.

关 键 词：制备工艺 板蓝根 多糖 淋巴细胞增殖 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]