反相高效液相色谱法测定复方鸡骨草片中栀子苷的含量  

RP-HPLC Method for the Assay of Jasminoidin in Compound Frutex Abri Tablets

作　　者：尹桂华[1] 杨美林[2] 仲崇林[2] 刘诗月[2] 

机构地区：[1]北华大学附属医院,吉林市132001 [2]吉林省中医中药研究院,长春市130021

出　　处：《中国药房》2007年第3期210-211,共2页China Pharmacy

摘　　要：目的：建立以反相高效液相色谱法测定复方鸡骨草片中栀子苷含量的方法。方法：色谱柱为Diamonsil C18，流动相为甲醇-0．1％磷酸溶液（22：78），流速为1．0mL·min^-1，检测波长为254nm，柱温为室温。结果：栀子苷进样量在0．125-0.623μg范围内线性关系良好（r=0．9996），平均回收率为97．81％（RSD=0．90％，n=9）。结论：本方法简便、准确、重现性好，可用于复方鸡骨草片的质量控制。OBJECTIVE： To establish a RP-HPLC method for the assay of Jasminoidin in compound frutex abri tablets METHODS： The separation of Jasminoldin was carried out on Diamonsil C18 under room temperature. The mobile phase consisted of methanol- 0. 1%H3PO4 solution（22 ： 78） at a flow rate of 1.0mL·min^-1. The detection wavelength was 254 nm. RESULTS： The linear range for Jasminoidin was 0.125-0.623μg（r = 0.999 6）, and the average recovery was 97.81% （RSD = 0.90 %, n = 9）.CONCLUSION ： The method is simple, accurate and reproducible, and suitable for the quality control of Jasminoidin in compound frutex abri tablets.

关 键 词：复方鸡骨草片 栀子苷 反相高效液相色谱法 含量测定 

分 类 号：R283.64[医药卫生—中药学] R927.2[医药卫生—中医学]