空间环境对黑色素瘤B16细胞体内增殖及免疫原性的影响  

Effects of Space Environment on Proliferation Ability in vivo and Immunogenicity of Melanoma B16 Cells

作　　者：耿传营[1] 向青[1] 徐梅[1] 郭宇鹏[2] 李红艳[1] 徐波[1] 房青[1] 刘玉凤[3] 唐劲天[3] 

机构地区：[1]中日友好临床医学研究所生物化学-分子生物学研究室,北京100029 [2]清华大学玉泉医院,北京100049 [3]清华大学工程物理系医学物理与工程研究所,北京100084

出　　处：《科技导报》2007年第1期24-27,共4页Science & Technology Review

基　　金：国家自然科学基金项目(30371324);科学技术部973计划前期项目[2003(CA04200)]

摘　　要：初步探讨空间环境对黑色素瘤B16细胞增殖及免疫原性的影响。选择4株经体外实验筛选出生物学性状变异明显的第20颗返回式卫星搭载的空间培养B16细胞,检测其体内增殖能力及免疫原性的变化。将筛选出的4株空间培养B16细胞接种于C57BL/6小鼠,其中一组接种于腹腔,观察荷瘤小鼠生存期;另一组接种于腋下皮肤,检测小鼠出瘤时间。接种至2周时,处死,分离荷瘤小鼠移植瘤和血清。称重荷瘤小鼠移植瘤,观察空间培养B16细胞增殖能力;利用HE染色法观察荷瘤小鼠移植瘤切片细胞形态;采用放射免疫分析法(Radioimmunoassay,RIA)测定荷瘤小鼠血清白介素-2(InterLeukin-2,IL-2)浓度,观察空间培养B16细胞免疫原性变化情况。与对照细胞相比,1株空间培养B16细胞的荷瘤小鼠生存期明显缩短,出瘤时间明显延长。HE结果显示:此株空间培养B16肿瘤组织内有淋巴细胞浸润;RIA结果显示:3株空间培养B16细胞荷瘤小鼠血清IL-2浓度明显增加。空间环境的复合因素可诱导B16细胞增殖能力及免疫原性产生变化。The effects of space environment on the proliferation ability and immunogenicity of melanoma B16 ceils are studied in this paper. Four B16 cells, which were on board in the 20th recoverable satellite, are selected. Their biological characteristics are found changed significantly in experiments in vitro. B16 cells selected were inoculated into C57BL/6 mice; each kind of space cells was injected into 20 mice, which were divided into two groups randomly, with＇ ten mice in each group inoculated with experimental ceils and one group were inoculated in the abdominal cavities to observe the survival time of tumor mice, and the other group were inoculated under the chest skins to record the time named as the forming-tumor time when the diameters of tumors exceeded 3 ram. The tumor mice were killed 2 weeks after the space cells were inoculated. Then, tumors were ablated and serum was separated. The tumors were weighted to measure the abilities of space mutant B16 cells in forming tumors; tumor slices were stained to observe the changes of shape of space mutant B16 cells; the concentration of InterLeukin-2 （IL-2） of the serum of tumor mice was determinrd to observe the immunogenicity of space cells with the method of radioimmunoassay （RIA）. Compared with wild melanoma B 16 cells, the survival time of the tumor mice with inoculated 11^# cells was found to be shortened significantly, while that with 4^#, 9^#, 15^# and Gro cells saw no apparent changes; the time in forming tumors for tumor mice inoculated with 11^# and Gro cells was prolonged significantly, while that with 4^#, 9^# and 15^# cells saw no apparent changes tumor mice inoculated with 11^# cell decreased significantly, that with 15^# cells increased, while that wi ; the weight of carcinomas of th 4^#, 9^# and Gro cell saw no apparent changes. Tumor slices stained by the method of HE showed that the necrotic area of carcinomas of 11^# space cells was reduced and there were lymphocytes within the tumor tissues in tumor mice inoculated with 11^# and 15^# sp

关 键 词：空间环境 黑素瘤 B16 增殖 免疫原性 

分 类 号：R85[医药卫生—航空、航天与航海医学]