极大螺旋藻胞内多糖对体外肿瘤细胞抑制作用的实验研究  

Effect of polysaccharides from Spirulina maxima on the growth of human tumor cell lines

作　　者：朱劲华[1] 张威[1] 王敏[2] 李朝军[2] 张成武[2] 

机构地区：[1]江苏职工医科大学,江苏南京210029 [2]南京师范大学江苏省分子医学生物技术重点实验室,江苏南京210097

出　　处：《海洋科学》2007年第1期1-5,共5页Marine Sciences

摘　　要：研究了极大螺旋藻(Spirulina maxima)胞内多糖对SMMC7721等肿瘤细胞系的生长及凋亡的影响。在体外培养条件下,用半抑制浓度(80,5 mg/L)的极大螺旋藻胞内多糖(IPSⅠ,IPSⅡ)处理肿瘤细胞,并用MTT,SRB,DNA ladder,AnnexinⅤ等方法测定。结果表明,IPSⅠ(80 mg/L)对SMMC7721,A549,HeLa,U251等肿瘤细胞系抑制率分别为64.97%、46.1%、43.3%、27.02%;IPSⅡ(5 mg/L)能诱导SMMC7721细胞凋亡。we observed the effect of inopolysaccharide of Spirulina maxima（IPS）on the growth and apoptosis of SMMC7721,A549,HeLa, U251 tumor cell lines.In vitro,human tumor cells were cultured in IPS Ⅰ and IPS Ⅱwith concentrations of 80 mg/L and 5 mg/L, the result of which was determined by MTT, SRB cell morphology, armexin Ⅴ apoptosis assay and DNA fragmentation. IPS Ⅰ （80 mg/L）displayed strong growth inhibitory effects on SMMC7721, A549,HeLa, U25 lhurnan cancer cell lines with 64.97%,46.1%,43.3%,27.02% of inhibition rate,respectively. IPS Ⅱ （5 mg/L） inducing the apoptosis of SMMC7721.IPS can significantly restrain the growth of human tumor cell lines and induce apoptosis.

关 键 词：极大螺旋藻(Spirulinamaxima) 胞内多糖 细胞凋亡 

分 类 号：Q2[生物学—细胞生物学]