压电蛋白传感芯片制备技术的研究  

Research on preparation technique of piezoelectric protein sensing chip

作　　者：温颖[1] 单桂秋[1] 刘仲明[1] 

机构地区：[1]广州军区广州总医院,广东广州510010

出　　处：《传感器与微系统》2007年第1期18-20,共3页Transducer and Microsystem Technologies

基　　金：广东省社会发展计划资助项目(2005B33101011)

摘　　要：在石英晶体微天平上用戊二醛交联法固定HBsAb,构建乙肝表面抗原(HBsAg)压电蛋白传感芯片,运用抽检、测试、数据统计分析、正交试验设计和质量检验等方法对芯片制作中的清洗、固定、封阻、检测等步骤进行质量控制,压电蛋白传感芯片稳定性、合格率均有显著提高。对压电蛋白传感芯片制备工艺进行质量控制,是提高芯片技术性能的关键。Piezoelectric protein sensing chip is constructed with immobilizing HBsAg probes onto the silver electrodes of quartz-crystal microbalance, which is modified with Glu cross-linked method to detect HBsAg. By random sampling inspection, data statistics, interactive experimeni and quality examination, quality control Of washing,immobilizing,seal and detection steps ,the stability, qualified and accurate percentage of sensing chip are developed. Quality control of preparation technique is the key to develop technique performance Of the piezoelectric protein sensing chip.

关 键 词：压电蛋白传感芯片 质量控制 乙肝表面抗原 

分 类 号：TP212.9[自动化与计算机技术—检测技术与自动化装置]