7种氨基醇类药物在替考拉宁柱上的对映体分离  

Enantioseparation of Seven Amino Alcohols on Teicoplanin Chiral Column

作　　者：徐贝佳[1] 张大同[1] 沈报春[1] 徐秀珠[1] 

机构地区：[1]浙江大学化学系,杭州310027

出　　处：《分析化学》2007年第1期55-60,共6页Chinese Journal of Analytical Chemistry

基　　金：浙江省科技计划资助项目(No2004C33050)

摘　　要：在替考拉宁手性柱上对阿替洛尔、美托洛尔、富马酸比索洛尔、普萘洛尔、异丙肾上腺素、沙丁胺醇及拉贝洛尔7种氨基醇类药物进行了对映体分离的研究。考察了流动相甲醇中添加少量酸碱以及酸碱不同比例对7种溶质的对映体分离的影响。固定极性有机相甲醇中冰醋酸和三乙胺的比例为1∶1时,手性溶质的保留因子均随着流动相中冰醋酸/三乙胺含量的增加而减小,分离因子(α)基本保持不变,分离度减小。这可能是因为过量的酸增加了流动相的极性,使溶质较快被洗脱。在不加冰醋酸的情况下,除拉贝洛尔外,随着三乙胺浓度的增加,容量因子均减少,分离因子基本不变,分离度减小。这可能是因为三乙胺的增加使流动相的极性增加,溶质与手性固定相间的氢键减弱。结果表明:冰醋酸和三乙胺的比例并不影响手性分离的实质。Under polar organic mode, the seven amino alcohols propranolol, bisoprolol fumarate, atenolol, salbutamol, isoprenaline, metoprolol and labetalol were enationseparated on teicoplanin chiral stationary phase （CSP）, using methanol as mobile phase and HOAc and triethylamine （TEA） as mobile phase additives. When the proportion of HOAc and TEA was 1 ： 1, the retention factors and the resolutions of seven amino alcohols were decreased with the increase of HOAc/TEA concentration, separation factors kept constant. The excessive acid increased the polarity of mobile phase, the eluting ability was increased. Without HOAc additive, the retention factors of the amino alcohols were decreased with increase of the amount TEA, separation factors kept constant, except labetalol. The polarity of mobile phase increased with the increasing of TEA concentration, so the hydrogen bonding between solutes and CSPs decreased. All the results show that the proportion of HOAc and TEA doesn＇t change the essential of chiral separation.

关 键 词：高效液相色谱 对映体分离 替考拉宁手性柱 氨基醇 机理研究 

分 类 号：R91[医药卫生—药学]