聚L-精氨酸修饰电极存在下同时测定多巴胺和肾上腺素  

Simultaneous Determination of Epinephrine and Dopamineat with Poly(L-arginine) Modified Electrode

作　　者：马伟[1] 孙登明[1] 

机构地区：[1]淮北煤炭师范学院化学系,淮北235000

出　　处：《分析化学》2007年第1期66-70,共5页Chinese Journal of Analytical Chemistry

基　　金：安徽省教育厅自然科学基金(No2004KJ320;2005KJ140);安徽省绿色化学重点实验室项目基金(NoKLSF(I)07);淮北市科技计划基金(No040145)资助项目

摘　　要：用循环伏安法制备了聚L-精氨酸修饰玻碳电极，研究了多巴胺和肾上腺素在修饰电极上的电化学行为。建立了同时测定多巴胺和肾上腺素的新方法。在pH7．5的磷酸盐缓冲溶液中。多巴胺在修饰电极上产生一对氧化还原峰，峰电位分别为0．276V和0．059V；肾上腺素在修饰电极上产生3个氧化峰和一个还原峰，峰电位分别为0．262V、0．121V、-0．126V和-0．316V（对Ag／AgCl电极）。多巴胺和肾上腺素同时存在时△Epc=375mV，用还原峰对多巴胺和。肾上腺素同时测定的线性范围分别为8．0×10^-7～5．0×10^-4mol／L和5．0×10^-7～5．0×10^-5mol／L；检出限分别为3．0×10^-7mol／L和1．0×10^-7mol／L。大量的抗坏血酸和尿酸不干扰测定，用于人尿液中多巴胺和。肾上腺素样品的同时测定，结果满意。A poly （L-arginine） chemical modified electrode（PLA/CME） was fabricated by electrochemical immobilization of L-arginine on a glassy carbon electrode （GCE） through cyclic vohammetry （CV）. Simultaneous determination of dopamine （DA） and epinephrine （EP） has been studied at PLA/GCE. The PLA/GCE exhibited superior selectivity and sensitivity towards DA and EP in the presence of high concentrations of uric acid （UA） and ascorbic acid （AA）. In pH 7.5 phosphate buffer solution, the modified electrode gave a pair of redox peaks at 0. 276 V and 0. 059 V for DA, three oxdition peaks and one cathodic peaks at 0. 262 V, 0. 121 V, -0. 126 V and -0. 316 V for EP, respectively. Two separated cathodic peaks was divided about 375 inV. The linear response were obtained in the range of 5.0 × 10^ -7 to 5.0 × 10^ -5 mol/L for EP, and 8.0 × 10^ -7 ～ 5.0 × 10^ -4 moL/L for DA. The detection limit were 1.0 ×10^-7moL/L for EP and 3.0 × 10^ -7moL/L for DA. The method was successfully applied to the simultaneous determination of DA and EP in human urine samples. Electrochemical behavior of DA and EP on the PLA/GCE were also investigated.

关 键 词：L-精氨酸 多巴胺 肾上腺素 化学修饰电极 同时测定 

分 类 号：R927[医药卫生—药学]