润肺口服液对慢性支气管炎大鼠支气管TNF-α、ICAM-1蛋白及IL-8表达的影响  

The effect of RunFei recipe on the protein expression of TNF-α,ICAM-1 and IL-8 in the bronchus of rats with CB

作　　者：刘艳波[1] 沈维高[2] 芦丽莉[2] 

机构地区：[1]吉林大学前列腺疾病防治研究中心 [2]北华大学医学院,吉林吉林132013

出　　处：《中国老年学杂志》2007年第1期8-11,共4页Chinese Journal of Gerontology

基　　金：吉林省科技厅科研基金项目(2004031-09)

摘　　要：目的研究中药复方润肺口服液对慢性支气管炎(CB,CB)支气管肿瘤坏死因子(TNF-α)、细胞间黏附分子-1(ICAM-1)及白介素-8(IL-8)表达的影响,探讨其治疗CB的疗效机制。方法雄性W istar大鼠随机分为假手术组、慢性支气管炎组、慢性支气管炎氢化可的松治疗组及慢性支气管炎润肺口服液治疗组,每组8只。大鼠慢性支气管炎模型通过熏香烟加气管内注入低剂量脂多糖制成,利用免疫组织化学观察各组大鼠支气管TNF-α、ICAM-1蛋白及IL-8的表达情况。结果熏香烟加气管内低剂量脂多糖注射可复制出较理想的慢性支气管炎模型,假手术组支气管上皮内可见TNF-α、ICAM-1蛋白及IL-8弱阳性表达;慢性支气管炎组支气管TNF-α、ICAM-1蛋白及IL-8在支气管上皮细胞、支气管周围的淋巴滤泡炎性细胞和肺泡间质细胞中可见强阳性表达;半定量图像分析显示,慢性支气管炎组TNF-α、ICAM-1蛋白及IL-8的表达明显强于假手术组(P<0.05),慢性支气管炎润肺口服液治疗组的表达则明显弱于支气管炎组(P<0.05)。结论润肺口服液通过下调支气管上皮细胞中TNFα-、ICAM-1蛋白及IL-8的表达,从而减轻气道炎症反应是其治疗慢性支气管炎的机制之一。Objective To research the effect of RunFei recipe on the protein of TNF-α, ICAM-1 and IL-8 in the bronchus of rats with CB and explore the therapeutic mechanism. Methods 24 male Wistar rats were randomly divided into sham operation group,chronic bronchitis （CB） group, CB hydrocortisone group and CB RunFei recipe group, $ rats in each group. The animal models were built up by passive cigarette smoking and intratracheal instillation of low dose lipopolysacchide to detect protein expression of TNF-α, ICAM-1 and IL-8 by immunochemical method in bronchus of rats. Results Using passive cigarette smoking and intratracheal instillation of low dose lipopo- lysacchide may reproduce ideal CB model. The expression of TNF-α, ICAM-1 and IL-8 were weak in the area of bronchial epithelium in sham operation group. While TNF-α, ICAM-1 and IL-8 were strongly expressed in bronchial epithelium, among the lymph follicular inflammatory cells and in the alveolar interstitial cells. Semi-fixed quantity analysis showed that the expression of TNF-α, ICAM-1 and IL-8 were stronger in CB group than those of sham operation group obviously（ P 〈0. 05 ） while the expression in CB RunFei recipe group were weaker than that of CB group （ P 〈 0. 05 ）. Conclusions Downregulating the expression of TNF-α, ICAM-1 and IL-8 in the area of bronchial epithelium may be one of the mechanisms underlying therapeutic effect of RunFei recipe on CB.

关 键 词：支气管炎 脂多糖 大鼠 肿瘤坏死因子 白细胞介素-8 

分 类 号：R562.21[医药卫生—呼吸系统]