枫香槲寄生总皂苷提取工艺研究及含量测定  

Studies on the Extraction Process and Determination of Total Saponins Content from Viscum liquidambaricolum Hayata

作　　者：杨燕军[1] 邓永燕[2] 赵南[3] 沙聪威[4] 

机构地区：[1]广东化工制药职业技术学院,广州510520 [2]广东药学院,2006届毕业实习生广州510006 [3]江西新余高等专科学校,江西新余338000 [4]广东省药物研究所,广州510440

出　　处：《广州中医药大学学报》2007年第1期62-65,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(编号:2002A301030205);广东省中医药局科研课题(编号:1040129;1050041)

摘　　要：【目的】研究枫香槲寄生总皂苷的最佳提取工艺并测定总皂苷的含量。【方法】采用正交试验法优选最佳提取工艺,以齐墩果酸为对照品通过比色法测定总皂苷的含量。【结果】最佳提取条件为10倍量体积分数为70%的乙醇回流提取3次,每次1 h。显色后的样品稳定,测定方法准确、重现性好,可用于该植物药总皂苷的含量测定。其中第3批药材总皂苷含量最高,其百分含量为8.16%。【结论】该提取工艺操作简单,经济省时,方便可行。[ Objective] To optimize the extraction process of total saponins from Viscum liquidambaricolum Hayata. and to assay the content of total saponins. [ Methods] Orthogonal test was used to optimize the extraction process. With oleanolic acid as the control, the content of total saponins was detected by chromatometry. [ Results ] The optimal extraction process was as follows： extraction with 70% ethanol in 10 times volume as much as the medicinal material, 3 times, one hour each time. The content of total saponins from the third batch was the highest, being 8.16%. [ Conclusion] The extraction process and content determination method for Viscum liguidambaricolum Hayata. is simple and effective.

关 键 词：枫香槲寄生/化学 总皂苷/分析 色谱法 

分 类 号：R284.1[医药卫生—中药学]