ZmC_4Ppc基因水稻的标记辅助选择及其后代的产量性状分析  

Marker-Assisted Selection of ZmC_4Ppc Transformed Rice and Their Performance of Yield Components

作　　者：向珣朝[1,2,3] 李季航[4,3] 何立斌[4,3] 李平[4,3] 

机构地区：[1]西南科技大学生命科学与工程学院 [2]四川农业大学水稻研究所,四川温江611130 [3]四川农业大学作物基因资源与遗传改良教育部重点实验室,四川雅安625014 [4]四川农业大学水稻研究所

出　　处：《中国水稻科学》2007年第1期25-30,共6页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2003AA212030);教育部长江学者和创新团队发展计划资助项目(IRT0453)。

摘　　要：ZmC4Ppc基因全长6781bp,PCR扩增时带型不清晰,重复性差,影响标记辅助选择的准确性。通过序列比对找到了该基因在玉米上存在而在水稻上不存在的特异片段,利用Primer Premier5.0设计了1对用于水稻标记辅助选择的特异性引物(前引物为5′AAGCAGGGAAGCGAGACG3′,后引物为5′GATTGCCGCCAGCAGTAG3′,命名为MRpc)。结果表明,经MRpc筛选的该基因在各生育阶段都能高效表达,且为组成性表达。对标记辅助选择后代FPM881进行了遗传背景、PEPCase活性、净光合速率、一般配合力(GCA)和特殊配合力(SCA)分析。FPM881与对照的遗传背景相似度已达95%以上,ZmC_4Ppc在MAS的各世代中能够高效表达。GCA和SCA分析结果表明从FPM881中能够筛选出单株产量、有效穗和千粒重优于对照、GCA较高的改良恢复系并得到SCA显著高于对照的杂交组合。试验结果说明,ZmC4Ppc基因高效表达能提高经济产量,在水稻遗传背景中发挥其基因效应。The full-length of intact ZmC4Ppc gene is 6781 bp, and it＇s difficult to obtain clear bands amplified by PCR, which affected the accuracy of marker-assisted selection （MAS） both in rice and maize. Specific fragments presented only in maize rather than in rice were identified and used for primer design using Primer Premier 5.0. A pair of specific primer termed MRpc （forward： 5＇ AAGCAGGGAAGCGAGACG 3＇, reverse： 5＇ GATTGCCGCCAGCAGTAG 3＇） was used for selection of transformed rice and ZmC4Ppc gene could be highly eonsitutive expressed at every developmental stage in rice. MRpc was used for MAS of progenies carrying ZINC4 Ppc gene in rice and a transformed rice line FPM881 was gained, which was tested for analyzing the genetic background, PEPCase activity, net photosynthetic rate （Pn）, general combining ability （GCA） and specific combining ability （SCA）. The results indicated that the FPM881 showed an above 95% similar genetic background, a higher expression of PEPCase and an improved net photosynthetic rate than those of control. Analysis of yield components showed that some of the progenies carrying the ZmC4 Ppc gene had better GCA and SCA for grain yield per plant, number of panicles per plant, and 1000-grain weight compared to the control. It suggested that the introduction of maize ZmC4Ppc gene via MAS with stable expression could increase rice grain yield and would likely provide a pathway for rice improvement.

关 键 词：ZmC4Ppc基因 特异性引物 标记辅助选择 水稻 玉米 

分 类 号：S511[农业科学—作物学]