新疆伊吾湖嗜盐菌bop基因多样性  

Bop gene diversity of Yiwu Lake in Xinjiang.

作　　者：古丽巴哈尔.阿巴拜克利 吴敏[2] 吴月红[2] 邵娇芳[2] 

机构地区：[1]新疆师范大学生命与环境科学学院生物系,新疆乌鲁木齐830054 [2]浙江大学生命科学学院,浙江杭州310027

出　　处：《浙江大学学报（理学版）》2007年第1期80-84,共5页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(编号:30370029);国家重点基础研究发展规划资助项目(973计划)子项目(编号:2004CB719604-3)

摘　　要：为了研究新疆中部地区伊吾湖嗜盐微生物和细菌视蛋白基因(bop)资源,分离纯化了95株嗜盐和耐盐菌.并从中挑选了10株红色的菌株,采用PCR和TA克隆的方法,扩增出bop基因螺旋C到螺旋G的核心序列,测定了基因的核苷酸序列.基于bop基因序列的同源性比较和系统发育学分析,发现9个序列分布在同一分支下,表明伊吾湖嗜盐菌bop基因具有一定的多样性和明显的自身地域性.In order to study the halophiles and bacterio-opsin protein （bop） gene resource from Yiwu Lake located in Xinjiang Region, ninety-five halopbilic strains were isolated. Ten red strains from the isolates were picked out and the genes encoding for bacterio-opsin protein fragment were proliferated by PCR, Their nucleotide sequences were determined subsequently. On the basis of phylogenetic analysis of the bop gene sequences,it was found that nine sequences clustered together. It is indicated that the bop genes of halophilic archaea from Yiwu lake have some diversity and terrain traits.

关 键 词：伊吾湖 嗜盐菌 细菌视蛋白基因 多样性 

分 类 号：Q939[生物学—微生物学]